| RRC ID |
42379
|
| Author |
Sano M, Furuta T, Takahira K, Kajimura M, Hanai H, Kohno E, Hirano T, Hishida A.
|
| Title |
Cell-cycle-dependent efficacy of photodynamic therapy with ATX-S10(Na).
|
| Journal |
Lasers Med Sci
|
| Abstract |
Photodynamic therapy (PDT) is a useful strategy for treating various cancers. Details of the mechanisms of PDT have not been made clear yet. We intended to study the efficacy of PDT in relation to the cell cycle. HeLa S3 cells were synchronized by the thymidine block method. Cells in different cell cycle phases after release were treated with the water-soluble photosensitizer, ATX-S10(Na). The cellular viability after PDT was determined by the MTT assay. Intracellular levels of ATX-S10(Na) in different cell cycle phases were also determined. We found that cells in the S and G(2)/M phases were hypersensitive to PDT with ATX-S10(Na) in comparison with those in the G(1) phase, and that cellular levels of ATX-S10(Na) were increased in cells in the S and G(2)/M phases compared to those in the G(1) phase. We conclude that cellular ATX-S10(Na) levels differ among the different cell cycle phases, which is associated with the cell-cycle-dependent efficacy of PDT with ATX-S10(Na).
|
| Volume |
20(1)
|
| Pages |
1-5
|
| Published |
2005-1-1
|
| DOI |
10.1007/s10103-005-0337-y
|
| PMID |
15912266
|
| MeSH |
Analysis of Variance
Cell Cycle / drug effects*
Female
HeLa Cells / cytology
HeLa Cells / drug effects*
Humans
In Vitro Techniques
Photochemotherapy / methods*
Porphyrins / pharmacology*
Probability
Sensitivity and Specificity
Tumor Cells, Cultured / cytology
Tumor Cells, Cultured / drug effects
|
| IF |
2.342
|
| Times Cited |
4
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
SURGERY
|
| Resource |
| Human and Animal Cells |
HeLa.S3(RCB0191) |
