| RRC ID |
42385
|
| Author |
Tanaka H, Tanabe N, Suzuki N, Shoji M, Torigoe H, Sugaya A, Motohashi M, Maeno M.
|
| Title |
Nicotine affects mineralized nodule formation by the human osteosarcoma cell line Saos-2.
|
| Journal |
Life Sci
|
| Abstract |
Several in vitro and in vivo studies have indicated that tobacco smoking may be an important risk factor for the development and severity of inflammatory periodontal disease. In the present study, we examined the effect of nicotine on cell proliferation, alkaline phosphatase (ALPase) activity, mineralized nodule formation, and the expression of extracellular matrix proteins in the human osteosarcoma cell line Saos-2. The cells were cultured with Dulbecco's modified Eagle medium containing 10% fetal bovine serum with 0, 10(-4) M, and 10(-3) M nicotine for up to 14 days. Mineralized nodule formation was examined by alizarin red staining, and the calcium content in mineralized nodules was determined using a calcium E-test kit. The expression of extracellular matrix proteins was estimated by determining the levels of their mRNAs using the real-time polymerase chain reaction. Mineralized nodule formation and calcium content in mineralized nodules were remarkably suppressed by nicotine on days 10 and 14 of culture, respectively. ALPase activity as well as type I collagen and osteopontin expression also decreased in the presence of nicotine after 5, 10, and 14 days of culture, respectively. By contrast, the amount of bone sialoprotein increased during 14 days of culture with nicotine. These results suggest that nicotine suppresses osteogenesis through a decrease in ALPase and type I collagen production by osteoblasts.
|
| Volume |
77(18)
|
| Pages |
2273-84
|
| Published |
2005-9-16
|
| DOI |
10.1016/j.lfs.2005.02.022
|
| PII |
S0024-3205(05)00447-9
|
| PMID |
15946696
|
| MeSH |
Alkaline Phosphatase / metabolism
Calcification, Physiologic / drug effects*
Calcium / metabolism
Cell Line, Tumor
Cell Proliferation / drug effects*
DNA Primers
Extracellular Matrix Proteins / metabolism
Humans
Nicotine / toxicity*
Osteogenesis / drug effects*
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
|
| IF |
3.647
|
| Times Cited |
38
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
MEDICINE, RESEARCH & EXPERIMENTAL
|
| Resource |
| Human and Animal Cells |
Saos-2(RCB0428) |
