RRC ID 4239
Author Hellwig S, Bass BL.
Title A starvation-induced noncoding RNA modulates expression of Dicer-regulated genes.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Although much has been learned about short noncoding RNAs, long noncoding transcripts are largely uncharacterized. Here, we describe Caenorhabditis elegans rncs-1, a highly base-paired, 800-nucleotide noncoding RNA expressed in hypodermis and intestine. Transcription of rncs-1 is modulated in response to food supply. Although highly double-stranded, we show that rncs-1 RNA is not a substrate for Dicer because of branched structures at its termini. However, rncs-1 RNA inhibits Dicer cleavage of a second dsRNA in vitro, presumably by competition. We validate this observation in vivo by demonstrating that mRNA levels of several Dicer-regulated genes vary with changes in rncs-1 expression. Certain viruses express dsRNA to compete with cellular dsRNA-mediated pathways, and our data suggest that rncs-1 provides a cellular correlate of this phenomenon.
Volume 105(35)
Pages 12897-902
Published 2008-9-2
DOI 10.1073/pnas.0805118105
PII 0805118105
PMID 18723671
PMC PMC2519042
MeSH Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics* Food Deprivation* Gene Expression Regulation* Genes, Helminth Genome Intestinal Mucosa / metabolism RNA Processing, Post-Transcriptional RNA, Small Interfering / metabolism RNA, Untranslated / genetics* Ribonuclease III / metabolism* Subcutaneous Tissue / metabolism Transcription, Genetic
IF 9.58
Times Cited 40
C.elegans tm1632