論文 - 詳細
| RRC ID | 4239 |
|---|---|
| 著者 | Hellwig S, Bass BL. |
| タイトル | A starvation-induced noncoding RNA modulates expression of Dicer-regulated genes. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Although much has been learned about short noncoding RNAs, long noncoding transcripts are largely uncharacterized. Here, we describe Caenorhabditis elegans rncs-1, a highly base-paired, 800-nucleotide noncoding RNA expressed in hypodermis and intestine. Transcription of rncs-1 is modulated in response to food supply. Although highly double-stranded, we show that rncs-1 RNA is not a substrate for Dicer because of branched structures at its termini. However, rncs-1 RNA inhibits Dicer cleavage of a second dsRNA in vitro, presumably by competition. We validate this observation in vivo by demonstrating that mRNA levels of several Dicer-regulated genes vary with changes in rncs-1 expression. Certain viruses express dsRNA to compete with cellular dsRNA-mediated pathways, and our data suggest that rncs-1 provides a cellular correlate of this phenomenon. |
| 巻・号 | 105(35) |
| ページ | 12897-902 |
| 公開日 | 2008-9-2 |
| DOI | 10.1073/pnas.0805118105 |
| PII | 0805118105 |
| PMID | 18723671 |
| PMC | PMC2519042 |
| MeSH | Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics* Food Deprivation* Gene Expression Regulation* Genes, Helminth Genome Intestinal Mucosa / metabolism RNA Processing, Post-Transcriptional RNA, Small Interfering / metabolism RNA, Untranslated / genetics* Ribonuclease III / metabolism* Subcutaneous Tissue / metabolism Transcription, Genetic |
| IF | 9.412 |
| 引用数 | 57 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1632 |