論文 - 詳細
| RRC ID | 42390 |
|---|---|
| 著者 | Hoshida Y, Kato N, Yoshida H, Wang Y, Tanaka M, Goto T, Otsuka M, Taniguchi H, Moriyama M, Imazeki F, Yokosuka O, Kawabe T, Shiratori Y, Omata M. |
| タイトル | Hepatitis C virus core protein and hepatitis activity are associated through transactivation of interleukin-8. |
| ジャーナル | J Infect Dis |
| Abstract |
BACKGROUND:We evaluated the association between variations in hepatitis C virus (HCV) core protein and hepatitis severity in patients with chronic HCV infection who achieved remission without viral eradication and had a biochemical response to interferon (IFN) therapy, to evaluate the effect of HCV core sequence in the absence of the influence of host factors. METHODS. Using serum from 10 patients with a biochemical response and 10 patients with no response, we measured serum levels of interleukin (IL)-1 beta , IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IFN- gamma , and tumor necrosis factor- alpha before and after IFN therapy. Expression vectors with the core region were transfected into Huh7 cells, and cytokine induction was evaluated by reporter assay. RESULTS:In biochemical responders, only IL-8 levels decreased after IFN therapy (P=.04). Changes in the C-terminal hydrophobic region were observed more frequently in biochemical responders. Activation of the IL-8 promoter by HCV core protein was significantly decreased in biochemical responders after IFN therapy (P=.04). When 69 C-terminal amino acids from before IFN therapy were replaced with those from after IFN therapy in 3 biochemical responders, their ability to transactivate IL-8 decreased. CONCLUSIONS:Differences in amino acids in the HCV core protein correlates with hepatitis activity through the modulation of IL-8 induction in HCV-infected patients. |
| 巻・号 | 192(2) |
| ページ | 266-75 |
| 公開日 | 2005-7-15 |
| DOI | 10.1086/430924 |
| PII | JID33740 |
| PMID | 15962221 |
| MeSH | Adult Amino Acid Sequence Female Genes, Reporter Genetic Variation Hepacivirus / genetics Hepacivirus / isolation & purification Hepatitis C / drug therapy Hepatitis C / immunology* Hepatitis C Antigens / genetics Humans Interferons / therapeutic use Interleukin-8 / genetics* Male Middle Aged Molecular Sequence Data Promoter Regions, Genetic Transcriptional Activation* Treatment Outcome Viral Core Proteins / chemistry Viral Core Proteins / genetics* |
| IF | 5.022 |
| 引用数 | 16 |
| WOS 分野 | INFECTIOUS DISEASES IMMUNOLOGY MICROBIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HuH-7 |