| RRC ID |
42394
|
| 著者 |
Seike M, Kondo T, Fujii K, Okano T, Yamada T, Matsuno Y, Gemma A, Kudoh S, Hirohashi S.
|
| タイトル |
Proteomic signatures for histological types of lung cancer.
|
| ジャーナル |
Proteomics
|
| Abstract |
We performed proteomic studies on lung cancer cells to elucidate the mechanisms that determine histological phenotype. Thirty lung cancer cell lines with three different histological backgrounds (squamous cell carcinoma, small cell lung carcinoma and adenocarcinoma) were subjected to two-dimensional difference gel electrophoresis (2-D DIGE) and grouped by multivariate analyses on the basis of their protein expression profiles. 2-D DIGE achieves more accurate quantification of protein expression by using highly sensitive fluorescence dyes to label the cysteine residues of proteins prior to two-dimensional polyacrylamide gel electrophoresis. We found that hierarchical clustering analysis and principal component analysis divided the cell lines according to their original histology. Spot ranking analysis using a support vector machine algorithm and unsupervised classification methods identified 32 protein spots essential for the classification. The proteins corresponding to the spots were identified by mass spectrometry. Next, lung cancer cells isolated from tumor tissue by laser microdissection were classified on the basis of the expression pattern of these 32 protein spots. Based on the expression profile of the 32 spots, the isolated cancer cells were categorized into three histological groups: the squamous cell carcinoma group, the adenocarcinoma group, and a group of carcinomas with other histological types. In conclusion, our results demonstrate the utility of quantitative proteomic analysis for molecular diagnosis and classification of lung cancer cells.
|
| 巻・号 |
5(11)
|
| ページ |
2939-48
|
| 公開日 |
2005-7-1
|
| DOI |
10.1002/pmic.200401166
|
| PMID |
15996008
|
| MeSH |
Adenocarcinoma / pathology
Biomarkers, Tumor
Carcinoma, Non-Small-Cell Lung / pathology
Carcinoma, Small Cell / pathology
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Electrophoresis, Polyacrylamide Gel
Humans
Lung / chemistry*
Lung / pathology
Lung Neoplasms / pathology*
Microdissection
Multivariate Analysis
Neoplasm Proteins / analysis*
Peptide Mapping
|
| IF |
3.254
|
| 引用数 |
36
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB0098)
PC-14(RCB0446)
RERF-LC-KJ(RCB1313)
LC-2/ad(RCB0440)
LC-1/sq(RCB0455)
LC-1F(RCB0439)
RERF-LC-AI(RCB0444)
Lu-130(RCB0465)
Lu-135(RCB0468)
Lu-139(RCB0469)
Lu-140(RCB0470)
Lu-165(RCB1184)
MS-1(RCB0725) |
