| RRC ID |
42406
|
| Author |
Ban S, Michikawa Y, Ishikawa K, Sagara M, Watanabe K, Shimada Y, Inazawa J, Imai T.
|
| Title |
Radiation sensitivities of 31 human oesophageal squamous cell carcinoma cell lines.
|
| Journal |
Int J Exp Pathol
|
| Abstract |
The purpose of this study was to determine the radiosensitivities of 31 human oesophageal squamous cell carcinoma cell lines with a colony-formation assay. A large variation in radiosensitivity existed among 31 cell lines. Such a large variation may partly explain the poor result of radiotherapy for this cancer. One cell line (KYSE190) demonstrated an unusual radiosensitivity. Ataxia-telangiectasia-mutated (ATM) gene in these cells had five missense mutations, and ATM protein was truncated or degraded. Inability to phosphorylate Chk2 in the irradiated KYSE190 cells suggests that the ATM protein in these cells had lost its function. The dysfunctional ATM protein may be a main cause of unusual radiosensitivity of KYSE190 cells. Because the donor of these cells was not diagnosed with ataxia telangiectasia, mutations in ATM gene might have occurred during the initiation and progression of cancer. Radiosensitive cancer developed in non-hereditary diseased patients must be a good target for radiotherapy.
|
| Volume |
86(4)
|
| Pages |
231-40
|
| Published |
2005-8-1
|
| DOI |
10.1111/j.0959-9673.2005.00431.x
|
| PII |
IEP431
|
| PMID |
16045545
|
| PMC |
PMC2517430
|
| MeSH |
Ataxia Telangiectasia Mutated Proteins
Blotting, Western / methods
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / radiotherapy*
Cell Cycle Proteins / genetics
Cell Line, Tumor
DNA Repair / genetics
DNA, Neoplasm / genetics
DNA-Activated Protein Kinase
DNA-Binding Proteins / genetics
Esophageal Neoplasms / genetics
Esophageal Neoplasms / radiotherapy*
Humans
Immunohistochemistry / methods
Mutation, Missense / genetics
Neoplasm Proteins / genetics
Nuclear Proteins
Phosphorylation
Protein Serine-Threonine Kinases / genetics
Radiation Tolerance / genetics
Tumor Suppressor Proteins / genetics
|
| IF |
1.672
|
| Times Cited |
8
|
|
WOS Category
|
PATHOLOGY
|
| Resource |
| Human and Animal Cells |
|
