| RRC ID |
42410
|
| 著者 |
Hansman GS, Matsubara N, Oka T, Ogawa S, Natori K, Takeda N, Katayama K.
|
| タイトル |
Deletion analysis of the sapovirus VP1 gene for the assembly of virus-like particles.
|
| ジャーナル |
Arch Virol
|
| Abstract |
Human sapovirus (SaV) strains are agents of gastroenteritis. They cannot be grown in cell culture. In this study, constructs containing SaV N- and C-terminal-deleted recombinant capsid proteins (rVP1) were expressed in a baculovirus expression system to allow us to better understand the sequence requirements for the formation of virus-like particles (VLPs). Only proteins derived from N-terminal-deleted rVP1 constructs that began 49 nucleotides downstream assembled into VLPs, which included both small and native-size VLPs. Our results were similar to those reported in a rabbit hemorrhagic disease virus (RHDV) N- and C-terminal-deleted rVP1 expression study but were distinct from those reported in a norovirus N- and C-terminal-deleted rVP1 expression study, suggesting that SaV and RHDV may have similar expression requirements.
|
| 巻・号 |
150(12)
|
| ページ |
2529-38
|
| 公開日 |
2005-12-1
|
| DOI |
10.1007/s00705-005-0599-5
|
| PMID |
16052282
|
| MeSH |
Baculoviridae
Capsid Proteins / genetics
Capsid Proteins / physiology*
Genetic Vectors
Hemorrhagic Disease Virus, Rabbit / genetics
Microscopy, Electron, Transmission
Norovirus / genetics
Sapovirus / genetics
Sapovirus / physiology*
Sequence Deletion*
Viral Proteins / genetics
Viral Proteins / physiology*
Viroids / ultrastructure
Virus Assembly / genetics
Virus Assembly / physiology*
|
| IF |
2.243
|
| 引用数 |
5
|
|
WOS 分野
|
VIROLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Sf9(RCB0563) |
