| RRC ID |
42440
|
| 著者 |
Hashimoto J, Ogawa T, Tsubota Y, Miyazaki K.
|
| タイトル |
Laminin-5 suppresses chondrogenic differentiation of murine teratocarcinoma cell line ATDC5.
|
| ジャーナル |
Exp Cell Res
|
| Abstract |
Laminin-5 is an important basement membrane protein that regulates cell adhesion and motility. It was previously found that the gamma2 chain of laminin-5 is transiently expressed in embryonic cartilage. This suggests a possible role of laminin-5 in chondrogenesis. Here, we examined this possibility using the murine teratocarcinoma cell line ATDC5. ATDC5 cells transiently and weakly expressed laminin-5 when they were stimulated for differentiation. Exogenous laminin-5 in either insoluble or soluble form strongly inhibited the differentiation phenotypes, i.e. formation of cartilaginous cell aggregates and production of chondrogenic marker proteins through its integrin-binding domain LG3 in the alpha3 chain. Laminin-5 had no effect on cell growth. In addition, we found that the laminin-5 with the 105-kDa, processed gamma2 chain suppressed differentiation more strongly than one with the 150-kDa gamma2 chain. This indicated that the proteolytic processing of gamma2 chain regulated the activity of laminin-5. However, a gamma2 chain short arm fragment had no effect on the chondrogenesis, and it rather suppressed the differentiation at excessive concentrations. These results suggest that laminin-5 and its processing modulate chondrogenic differentiation during development.
|
| 巻・号 |
310(2)
|
| ページ |
256-69
|
| 公開日 |
2005-11-1
|
| DOI |
10.1016/j.yexcr.2005.07.025
|
| PII |
S0014-4827(05)00339-3
|
| PMID |
16165127
|
| MeSH |
Animals
Cartilage / cytology
Cartilage / metabolism
Cell Adhesion Molecules / genetics
Cell Adhesion Molecules / pharmacology
Cell Adhesion Molecules / physiology*
Cell Differentiation
Cell Line, Tumor
Chondrocytes / cytology*
Mice
Protein Structure, Tertiary
Teratocarcinoma
|
| IF |
3.383
|
| 引用数 |
9
|
|
WOS 分野
|
ONCOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ATDC5(RCB0565) |
