論文 - 詳細
| RRC ID | 42440 |
|---|---|
| 著者 | Hashimoto J, Ogawa T, Tsubota Y, Miyazaki K. |
| タイトル | Laminin-5 suppresses chondrogenic differentiation of murine teratocarcinoma cell line ATDC5. |
| ジャーナル | Exp Cell Res |
| Abstract |
Laminin-5 is an important basement membrane protein that regulates cell adhesion and motility. It was previously found that the gamma2 chain of laminin-5 is transiently expressed in embryonic cartilage. This suggests a possible role of laminin-5 in chondrogenesis. Here, we examined this possibility using the murine teratocarcinoma cell line ATDC5. ATDC5 cells transiently and weakly expressed laminin-5 when they were stimulated for differentiation. Exogenous laminin-5 in either insoluble or soluble form strongly inhibited the differentiation phenotypes, i.e. formation of cartilaginous cell aggregates and production of chondrogenic marker proteins through its integrin-binding domain LG3 in the alpha3 chain. Laminin-5 had no effect on cell growth. In addition, we found that the laminin-5 with the 105-kDa, processed gamma2 chain suppressed differentiation more strongly than one with the 150-kDa gamma2 chain. This indicated that the proteolytic processing of gamma2 chain regulated the activity of laminin-5. However, a gamma2 chain short arm fragment had no effect on the chondrogenesis, and it rather suppressed the differentiation at excessive concentrations. These results suggest that laminin-5 and its processing modulate chondrogenic differentiation during development. |
| 巻・号 | 310(2) |
| ページ | 256-69 |
| 公開日 | 2005-11-1 |
| DOI | 10.1016/j.yexcr.2005.07.025 |
| PII | S0014-4827(05)00339-3 |
| PMID | 16165127 |
| MeSH | Animals Cartilage / cytology Cartilage / metabolism Cell Adhesion Molecules / genetics Cell Adhesion Molecules / pharmacology Cell Adhesion Molecules / physiology* Cell Differentiation Cell Line, Tumor Chondrocytes / cytology* Mice Protein Structure, Tertiary Teratocarcinoma |
| IF | 3.383 |
| 引用数 | 9 |
| WOS 分野 | ONCOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |