| RRC ID |
42469
|
| Author |
Ogra Y, Ishiwata K, Iwashita Y, Suzuki KT.
|
| Title |
Simultaneous speciation of selenium and sulfur species in selenized odorless garlic (Allium sativum L. Shiro) and shallot (Allium ascalonicum) by HPLC-inductively coupled plasma-(octopole reaction system)-mass spectrometry and electrospray ionization-tandem mass spectrometry.
|
| Journal |
J Chromatogr A
|
| Abstract |
The simultaneous speciation of selenium and sulfur in selenized odorless garlic (Allium sativum L. Shiro) and a weakly odorous Allium plant, shallot (Allium ascalonicum), was performed by means of a hyphenated technique, a HPLC coupled with an inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) equipped with an octopole reaction system (ORS). The aqueous extracts of them contained the common seleno compound that was identified as gamma-glutamylmethylselenocysteine by an electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Normal garlic contains alliin as the major sulfur-containing compound, which is the biological precursor of the garlic odorant, allicin. Alliin, however, was not detected in the extracts of the selenized odorless garlic. At least, four unidentified sulfur-containing compounds were detected in odorless garlic and shallot. Moreover, these Allium plants showed chemopreventive effects against human leukemia cells.
|
| Volume |
1093(1-2)
|
| Pages |
118-25
|
| Published |
2005-11-4
|
| DOI |
10.1016/j.chroma.2005.07.081
|
| PII |
S0021-9673(05)01577-3
|
| PMID |
16233877
|
| MeSH |
Allium / chemistry*
Amino Acids, Sulfur / analysis
Antineoplastic Agents, Phytogenic
Chromatography, High Pressure Liquid / methods*
HL-60 Cells
Humans
Mass Spectrometry / methods*
Selenium / analysis*
Sulfur / analysis*
|
| IF |
4.049
|
| Times Cited |
53
|
|
WOS Category
|
CHEMISTRY, ANALYTICAL
BIOCHEMICAL RESEARCH METHODS
|
| Resource |
| Human and Animal Cells |
HL60,(RCB0041) |
