Reference - Detail
| RRC ID | 42482 |
|---|---|
| Author | Mizoguchi I, Ooe Y, Hoshino S, Shimura M, Kasahara T, Kano S, Ohta T, Takaku F, Nakayama Y, Ishizaka Y. |
| Title | Improved gene expression in resting macrophages using an oligopeptide derived from Vpr of human immunodeficiency virus type-1. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Vpr, an accessory gene product of human immunodeficiency virus type-1, is thought to transport a viral DNA from the cytoplasm to the nucleus in resting macrophages. Previously, we reported that a peptide encompassing amino acids 52-78 of Vpr (C45D18) promotes the nuclear trafficking of recombinant proteins that are conjugated with C45D18. Here, we present evidence that C45D18, when conjugated with a six-branched cationic polymer of poly(N,N-dimethylaminopropylacrylamide)-block-oligo(4-aminostyrene) (SV: star vector), facilitates gene expression in resting macrophages. Although there was no difference between SV alone and C45D18-SV with respect to gene transduction into growing cells, C45D18-SV resulted in more than 40-fold greater expression of the exogenous gene upon transduction into chemically differentiated macrophages and human quiescent monocyte-derived macrophages. The data suggest that C45D18 contributes to improving the ability of a non-viral vector to transduce macrophages with exogenous genes and we discuss its further application. |
| Volume | 338(3) |
| Pages | 1499-506 |
| Published | 2005-12-23 |
| DOI | 10.1016/j.bbrc.2005.10.112 |
| PII | S0006-291X(05)02358-2 |
| PMID | 16274667 |
| MeSH | Amino Acid Sequence Cell Line, Tumor Cell Nucleus / chemistry Cell Nucleus / metabolism DNA / genetics Gene Expression / drug effects* Gene Expression / genetics Gene Products, vpr / chemistry* HIV-1 / chemistry* HIV-1 / genetics Humans Macrophages / drug effects* Macrophages / metabolism* Molecular Sequence Data Peptide Fragments / chemistry* Peptide Fragments / genetics Peptide Fragments / pharmacology* vpr Gene Products, Human Immunodeficiency Virus |
| IF | 2.985 |
| Times Cited | 16 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | THP-1(RCB1189) |