論文 - 詳細
| RRC ID | 42501 |
|---|---|
| 著者 | Oishi J, Kawamura K, Kang JH, Kodama K, Sonoda T, Murata M, Niidome T, Katayama Y. |
| タイトル | An intracellular kinase signal-responsive gene carrier for disordered cell-specific gene therapy. |
| ジャーナル | J Control Release |
| Abstract |
We have previously reported artificial gene-regulation systems responding to cyclic AMP-dependent protein kinase (PKA) using cationic polymer. This cationic polymer (PAK) was a graft-type polymer with an oligopeptide that is a substrate for PKA and could regulate gene-expression in a cell-free system. In the present study, we carried out a detailed characterization of the PAK-DNA complex (AFM observation and DLS measurement) and tried to apply this polymer to living cells. In the unstimulated NIH 3T3 cells, transfection of the PAK-DNA complex showed no expression of the delivered gene. This means that PAK formed a stable complex with DNA in the normal cells to totally suppress gene expression. In contrast, significant expression was seen when the PAK-DNA complex was delivered to forskolin-treated cells. Thus, activated PKA disintegrates the complexes even in living cells, resulting in gene expression. Our results indicate that this type of intracellular signal-responsive polymer will be useful for the cell-specific release of genes. |
| 巻・号 | 110(2) |
| ページ | 431-436 |
| 公開日 | 2006-1-10 |
| DOI | 10.1016/j.jconrel.2005.10.007 |
| PII | S0168-3659(05)00530-4 |
| PMID | 16309776 |
| MeSH | 3T3 Cells Adenosine Triphosphate / chemistry Animals Cyclic AMP-Dependent Protein Kinases / genetics* Cyclic AMP-Dependent Protein Kinases / metabolism DNA / biosynthesis DNA / genetics Drug Carriers* Genes, Reporter Genetic Therapy / methods* Green Fluorescent Proteins / genetics Light Luciferases / genetics Mice Microscopy, Atomic Force Peptides / chemistry Peptides / metabolism Phosphorylation Polymers / chemical synthesis Scattering, Radiation Tetrazolium Salts / chemistry |
| IF | 7.727 |
| 引用数 | 42 |
| WOS 分野 | PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH/3T3 |