| RRC ID |
42502
|
| 著者 |
Tsujita T, Ishii A, Tsukada H, Matsumoto M, Che FS, Seya T.
|
| タイトル |
Fish soluble Toll-like receptor (TLR)5 amplifies human TLR5 response via physical binding to flagellin.
|
| ジャーナル |
Vaccine
|
| Abstract |
Fish has a soluble form of TLR5 ortholog (TLR5S), which does not exist in mammals. We identified TLR5S from rainbow trout and named rtTLR5S, which was about 38% homologous to the extracellular domains of human (hu) and mouse TLR5. Adjuvancy of rtTLR5S to flagellin response by human TLR5 (huTLR5) was tested in this study. A chimera constructed of rtTLR5S and the intracellular TIR of huTLR5 expressed on HeLa cells signaled the presence of various species of bacterial flagellin resulting in NK-kappaB activation. huTLR5S, when co-expressed with rtTLR5S in HeLa cells, augmented response to flagellin resulting in robust huTLR5-mediated NF-kappaB activation. Physical binding of flagellin to rtTLR5S was detected under the conditions where huTLR5 induced rtTLR5S-amplified NF-kappaB activation. Signal amplification by rtTLR5S was specific to huTLR5: no other huTLRs tested were responded to rtTLR5S. These results suggest that the soluble TLR5 serves as an adjuvant augmenting flagellin-TLR5-mediated NF-kappaB activation even in human.
|
| 巻・号 |
24(12)
|
| ページ |
2193-9
|
| 公開日 |
2006-3-15
|
| DOI |
10.1016/j.vaccine.2005.11.003
|
| PII |
S0264-410X(05)01126-6
|
| PMID |
16314010
|
| MeSH |
Amino Acid Sequence
Animals
Flagellin / isolation & purification
Flagellin / metabolism*
HeLa Cells
Humans
Molecular Sequence Data
Oncorhynchus mykiss
Toll-Like Receptor 5 / chemistry*
Toll-Like Receptor 5 / genetics
Toll-Like Receptor 5 / metabolism*
|
| IF |
3.143
|
| 引用数 |
45
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
