論文 - 詳細
| RRC ID | 42502 |
|---|---|
| 著者 | Tsujita T, Ishii A, Tsukada H, Matsumoto M, Che FS, Seya T. |
| タイトル | Fish soluble Toll-like receptor (TLR)5 amplifies human TLR5 response via physical binding to flagellin. |
| ジャーナル | Vaccine |
| Abstract |
Fish has a soluble form of TLR5 ortholog (TLR5S), which does not exist in mammals. We identified TLR5S from rainbow trout and named rtTLR5S, which was about 38% homologous to the extracellular domains of human (hu) and mouse TLR5. Adjuvancy of rtTLR5S to flagellin response by human TLR5 (huTLR5) was tested in this study. A chimera constructed of rtTLR5S and the intracellular TIR of huTLR5 expressed on HeLa cells signaled the presence of various species of bacterial flagellin resulting in NK-kappaB activation. huTLR5S, when co-expressed with rtTLR5S in HeLa cells, augmented response to flagellin resulting in robust huTLR5-mediated NF-kappaB activation. Physical binding of flagellin to rtTLR5S was detected under the conditions where huTLR5 induced rtTLR5S-amplified NF-kappaB activation. Signal amplification by rtTLR5S was specific to huTLR5: no other huTLRs tested were responded to rtTLR5S. These results suggest that the soluble TLR5 serves as an adjuvant augmenting flagellin-TLR5-mediated NF-kappaB activation even in human. |
| 巻・号 | 24(12) |
| ページ | 2193-9 |
| 公開日 | 2006-3-15 |
| DOI | 10.1016/j.vaccine.2005.11.003 |
| PII | S0264-410X(05)01126-6 |
| PMID | 16314010 |
| MeSH | Amino Acid Sequence Animals Flagellin / isolation & purification Flagellin / metabolism* HeLa Cells Humans Molecular Sequence Data Oncorhynchus mykiss Toll-Like Receptor 5 / chemistry* Toll-Like Receptor 5 / genetics Toll-Like Receptor 5 / metabolism* |
| IF | 3.143 |
| 引用数 | 45 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |