Reference - Detail
| RRC ID | 42508 |
|---|---|
| Author | Takagi M, Suto F, Suga T, Yamada J. |
| Title | Sterol Regulatory Element-Binding Protein-2 modulates human brain acyl-CoA hydrolase gene transcription. |
| Journal | Mol Cell Biochem |
| Abstract |
The brain shows high catalyzing activity during hydrolysis of long-chain acyl-CoAs into fatty acids and CoA-SH. Brain acyl-CoA hydrolase (BACH) is responsible for most of the long-chain acyl-CoA hydrolyzing activity in the brain and is localized exclusively in neurons. We analyzed the human BACH gene promoter, focusing on transcriptional regulation by Sterol Regulatory Element-Binding Protein-2 (SREBP-2), which is a transcription factor that activates genes involved in cholesterol biosynthesis and uptake. When the nuclear form of SREBP-2 gene was transfected into human neuroblastoma cells, transcription of a BACH gene promoter-luciferase reporter gene was activated through a sterol regulatory element (SRE) motif. Moreover, a gel shift assay demonstrated that SREBP-2 specifically bound to the SRE motif. These results suggest that transcription of the BACH gene is activated by SREBP-2. This study also provides insights into BACH function in the interaction between the metabolism of acyl-CoAs and cholesterol in neurons. |
| Volume | 275(1-2) |
| Pages | 199-206 |
| Published | 2005-7-1 |
| DOI | 10.1007/s11010-005-1990-y |
| PMID | 16335799 |
| MeSH | Brain / enzymology* Cell Line, Tumor Electrophoretic Mobility Shift Assay Gene Expression Regulation Genes, Reporter HeLa Cells Humans Luciferases / metabolism Neuroblastoma / pathology Palmitoyl-CoA Hydrolase / genetics* Promoter Regions, Genetic Sterol Regulatory Element Binding Protein 2 / metabolism* Transcription, Genetic* Transfection |
| IF | 2.795 |
| Times Cited | 7 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |