| RRC ID |
42508
|
| Author |
Takagi M, Suto F, Suga T, Yamada J.
|
| Title |
Sterol Regulatory Element-Binding Protein-2 modulates human brain acyl-CoA hydrolase gene transcription.
|
| Journal |
Mol Cell Biochem
|
| Abstract |
The brain shows high catalyzing activity during hydrolysis of long-chain acyl-CoAs into fatty acids and CoA-SH. Brain acyl-CoA hydrolase (BACH) is responsible for most of the long-chain acyl-CoA hydrolyzing activity in the brain and is localized exclusively in neurons. We analyzed the human BACH gene promoter, focusing on transcriptional regulation by Sterol Regulatory Element-Binding Protein-2 (SREBP-2), which is a transcription factor that activates genes involved in cholesterol biosynthesis and uptake. When the nuclear form of SREBP-2 gene was transfected into human neuroblastoma cells, transcription of a BACH gene promoter-luciferase reporter gene was activated through a sterol regulatory element (SRE) motif. Moreover, a gel shift assay demonstrated that SREBP-2 specifically bound to the SRE motif. These results suggest that transcription of the BACH gene is activated by SREBP-2. This study also provides insights into BACH function in the interaction between the metabolism of acyl-CoAs and cholesterol in neurons.
|
| Volume |
275(1-2)
|
| Pages |
199-206
|
| Published |
2005-7-1
|
| DOI |
10.1007/s11010-005-1990-y
|
| PMID |
16335799
|
| MeSH |
Brain / enzymology*
Cell Line, Tumor
Electrophoretic Mobility Shift Assay
Gene Expression Regulation
Genes, Reporter
HeLa Cells
Humans
Luciferases / metabolism
Neuroblastoma / pathology
Palmitoyl-CoA Hydrolase / genetics*
Promoter Regions, Genetic
Sterol Regulatory Element Binding Protein 2 / metabolism*
Transcription, Genetic*
Transfection
|
| IF |
2.795
|
| Times Cited |
7
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
HeLa(RCB0007) |
