| RRC ID |
42519
|
| 著者 |
Islam S, Hassan F, Tumurkhuu G, Ito H, Koide N, Mori I, Yoshida T, Yokochi T.
|
| タイトル |
Lipopolysaccharide prevents apoptosis induced by brefeldin A, an endoplasmic reticulum stress agent, in RAW 264.7 cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
The effect of lipopolysaccharide (LPS) on the cell death induced by endoplasmic reticulum (ER) stress agents in RAW 264.7 cells was studied. LPS prevented the cell death by brefeldin A, but not thapsigargin and tunicamycin. CpG DNA as well as LPS prevented brefeldin A-induced cell death whereas tumor necrosis factor-alpha or interferon-gamma did not. Brefeldin A-induced cell death was mediated with apoptotic cell death and it was significantly inhibited by LPS. LPS abolished the activation of ER stress-related caspases, such as caspases 1, 3, and 4. LPS prevented brefeldin A-induced morphological changes in RAW 264.7 cells. Further, LPS prevented brefeldin A-induced Golgi dispersion. Therefore, LPS was suggested to diminish the stress of ER/Golgi complexes induced by brefeldin A and inhibit apoptosis. The preventive action of LPS on brefeldin A-induced apoptosis is discussed.
|
| 巻・号 |
340(2)
|
| ページ |
589-96
|
| 公開日 |
2006-2-10
|
| DOI |
10.1016/j.bbrc.2005.12.050
|
| PII |
S0006-291X(05)02801-9
|
| PMID |
16380090
|
| MeSH |
Animals
Apoptosis / drug effects*
Apoptosis / physiology
Brefeldin A / pharmacology*
Caspases / metabolism
Cell Line
Cell Survival / drug effects
Endoplasmic Reticulum / drug effects*
Endoplasmic Reticulum / metabolism
Golgi Apparatus / drug effects
Heme Oxygenase-1 / biosynthesis
Heme Oxygenase-1 / genetics
Lipopolysaccharides / pharmacology*
Macrophages / cytology
Macrophages / drug effects
Macrophages / metabolism
Mice
Oxidative Stress / drug effects*
Oxidative Stress / physiology
Thapsigargin / pharmacology
Tunicamycin / pharmacology
|
| IF |
2.985
|
| 引用数 |
14
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
