Reference - Detail
| RRC ID | 42521 |
|---|---|
| Author | Verbrugge A, Rijkers ES, de Ruiter T, Meyaard L. |
| Title | Leukocyte-associated Ig-like receptor-1 has SH2 domain-containing phosphatase-independent function and recruits C-terminal Src kinase. |
| Journal | Eur J Immunol |
| Abstract |
Most inhibitory receptors in the immune system contain one or several immunoreceptor tyrosine-based inhibitory motifs (ITIM) and recruit the SH2 domain-containing phosphatases SHP-1, SHP-2 and/or SHIP, which are generally believed to be essential for the inhibitory function. However, it has not been systematically investigated whether ITIM-bearing receptors exert their function through alternative interactions. Here we describe that leukocyte-associated Ig-like receptor (LAIR)-1 has inhibitory function in DT40 chicken B cells that lack both SHP-1 and SHP-2. In addition, we found that LAIR-1 did not recruit SHIP upon phosphorylation. Thus, LAIR-1 can function independently from SH2 domain-containing phosphatases and must recruit at least one other signaling molecule. Using a yeast-tri-hybrid system, we found that phosphorylated LAIR-1 bound the C-terminal Src kinase (Csk). The interaction required the SH2 domain of Csk and phosphorylation of the tyrosine in the N-terminal ITIM of LAIR-1. We propose that Csk is an additional player in the regulation of the immune system by ITIM-bearing receptors. |
| Volume | 36(1) |
| Pages | 190-8 |
| Published | 2006-1-1 |
| DOI | 10.1002/eji.200535226 |
| PMID | 16380958 |
| MeSH | Animals B-Lymphocytes / immunology* B-Lymphocytes / metabolism Blotting, Western CSK Tyrosine-Protein Kinase Cell Line Chickens Humans Mice Phosphoric Monoester Hydrolases Protein-Tyrosine Kinases / immunology Protein-Tyrosine Kinases / metabolism* Receptors, Antigen, B-Cell / immunology Receptors, Antigen, B-Cell / metabolism Receptors, Immunologic / immunology Receptors, Immunologic / metabolism* Two-Hybrid System Techniques src Homology Domains / immunology* src-Family Kinases |
| IF | 4.404 |
| Times Cited | 44 |
| WOS Category | IMMUNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | SHP1^(-) DT40(RCB1466) SHP2^(-) DT40(RCB1502) SHP1^(-)/SHP2^(-) DT40(RCB1501) SHIP^(-) DT40(RCB1645) |