Reference - Detail
| RRC ID | 42532 |
|---|---|
| Author | Matsubara T, Shirai Y, Miyasaka K, Murakami T, Yamaguchi Y, Ueyama T, Kai M, Sakane F, Kanoh H, Hashimoto T, Kamada S, Kikkawa U, Saito N. |
| Title | Nuclear transportation of diacylglycerol kinase gamma and its possible function in the nucleus. |
| Journal | J Biol Chem |
| Abstract |
Diacylglycerol kinases (DGKs) convert diacylglycerol (DG) to phosphatidic acid, and both lipids are known to play important roles in lipid signal transduction. Thereby, DGKs are considered to be a one of the key players in lipid signaling, but its physiological function remains to be solved. In an effort to investigate one of nine subtypes, we found that DGKgamma came to be localized in the nucleus with time in all cell lines tested while seen only in the cytoplasm at the early stage of culture, indicating that DGKgamma is transported from the cytoplasm to the nucleus. The nuclear transportation of DGKgamma didn't necessarily need DGK activity, but its C1 domain was indispensable, suggesting that the C1 domain of DGKgamma acts as a nuclear transport signal. Furthermore, to address the function of DGKgamma in the nucleus, we produced stable cell lines of wild-type DGKgamma and mutants, including kinase negative, and investigated their cell size, growth rate, and cell cycle. The cells expressing the kinase-negative mutant of DGKgamma were larger in size and showed slower growth rate, and the S phase of the cells was extended. These findings implicate that nuclear DGKgamma regulates cell cycle. |
| Volume | 281(10) |
| Pages | 6152-64 |
| Published | 2006-3-10 |
| DOI | 10.1074/jbc.M509873200 |
| PII | S0021-9258(19)57969-8 |
| PMID | 16407189 |
| MeSH | Active Transport, Cell Nucleus / genetics Active Transport, Cell Nucleus / physiology Animals CHO Cells COS Cells Cell Nucleus / enzymology* Cell Nucleus / genetics Cell Nucleus / physiology Cell Size Chlorocebus aethiops Cricetinae Cricetulus Diacylglycerol Kinase / genetics Diacylglycerol Kinase / metabolism* Diacylglycerol Kinase / physiology HeLa Cells Humans Isoenzymes / genetics Isoenzymes / metabolism Isoenzymes / physiology Mice NIH 3T3 Cells |
| IF | 4.238 |
| Times Cited | 29 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | COS-7(RCB0539) HeLa(RCB0007) |