RRC ID 42542
著者 Yokoyama H, Nakanishi H, Kodera Y, Ikehara Y, Ohashi N, Ito Y, Koike M, Fujiwara M, Tatematsu M, Nakao A.
タイトル Biological significance of isolated tumor cells and micrometastasis in lymph nodes evaluated using a green fluorescent protein-tagged human gastric cancer cell line.
ジャーナル Clin Cancer Res
Abstract PURPOSE:The biological significance of isolated tumor cells and micrometastasis in lymph node defined by the International Union against Cancer remains essentially unknown because of the lack of appropriate animal models. In the present study, we developed a lymph node micrometastasis model featuring a human gastric cancer cell line tagged with green fluorescent protein gene (GCIY-EGFP), which allows visualization of even isolated tumor cells in the development of metastasis without histologic procedure. Using this model, we investigated the effect of surgery and chemotherapy on the growth of early-phase metastasis formation in the lymph nodes.
EXPERIMENTAL DESIGN:The time course of spontaneous inguinal lymph node metastasis after s.c. inoculation of GCIY-EGFP cells into nude mice was examined with fluorescence dissecting microscopy. Then, the effects of surgical removal of the primary tumor with or without anti-asialo GM1 treatment or postoperative chemotherapy on the growth of isolated tumor cells and micrometastasis in the lymph nodes were examined.
RESULTS:GCIY-EGFP cells were found to metastasize spontaneously to the inguinal lymph nodes to form isolated tumor cells, micrometastasis, and, finally, develop macroscopic metastasis at 1 to 2, 3 to 5, and 5 weeks postinjection, respectively. When the primary tumors were removed within 2 weeks of inoculation, isolated tumor cells, but not micrometastasis, in the lymph nodes regressed by 4 weeks after surgery in all the mice examined (five of five). This spontaneous regression of isolated tumor cells was completely reversed by anti-asialo GM1 treatment, which could deplete natural killer cells effectively in nude mice. Chemotherapy following resection of the primary tumor at an early stage partially eliminated the remaining micrometastasis in the lymph nodes.
CONCLUSIONS:These results suggest that isolated tumor cells in the regional lymph nodes regressed by removal of the primary tumor mainly via natural killer cell-mediated antitumor activity and that micrometastasis in the lymph nodes could be effectively eliminated by the postoperative chemotherapy.
巻・号 12(2)
ページ 361-8
公開日 2006-1-15
DOI 10.1158/1078-0432.CCR-05-1963
PII 12/2/361
PMID 16428473
MeSH Animals Antibodies, Monoclonal / therapeutic use G(M1) Ganglioside / immunology Gastrectomy Green Fluorescent Proteins* Humans Killer Cells, Natural / immunology Lymph Node Excision Lymph Nodes Lymphatic Metastasis Male Mice Mice, Nude Neoplasm Staging Neoplasm Transplantation Postoperative Care Skin Neoplasms / pathology* Stomach Neoplasms / pathology* Tumor Cells, Cultured
IF 10.107
引用数 30
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 GCIY(RCB0555)