| RRC ID |
42548
|
| Author |
Tao RH, Kawate H, Ohnaka K, Ishizuka M, Hagiwara H, Takayanagi R.
|
| Title |
Opposite effects of alternative TZF spliced variants on androgen receptor.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
We previously demonstrated that testicular zinc-finger protein (TZF) was a corepressor of the androgen receptor (AR). In the present study, we further showed that TZF-L, an alternative spliced variant of TZF, enhanced transactivation function of AR. Deletion analysis of TZF-L revealed that its N-terminus, which almost corresponded to that of TZF, but not its C-terminus was able to interact with AR. Additional analysis suggested that TZF and TZF-L were able to form both homodimers and heterodimers. TZF-L inhibited the homodimer formation of TZF and the intranuclear dot formation of TZF. We propose that in the unique regulation system of AR-mediated transactivation, two spliced isoforms of TZF act as coactivator and corepressor, respectively.
|
| Volume |
341(2)
|
| Pages |
515-21
|
| Published |
2006-3-10
|
| DOI |
10.1016/j.bbrc.2005.12.213
|
| PII |
S0006-291X(06)00045-3
|
| PMID |
16446156
|
| MeSH |
Alternative Splicing*
Amino Acid Motifs
Animals
COS Cells
Cell Line, Tumor
Chlorocebus aethiops
Dimerization
Gene Deletion
Genes, Reporter
Humans
Immunoblotting
Immunoprecipitation
Mice
Microscopy, Confocal
Models, Biological
NIH 3T3 Cells
Plasmids / metabolism
Protein Isoforms
Protein Structure, Tertiary
Receptors, Androgen / metabolism*
Repressor Proteins / chemistry
Repressor Proteins / metabolism*
Transcriptional Activation
Transfection
|
| IF |
2.985
|
| Times Cited |
10
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
COS-7(RCB0539) |
