RRC ID |
42558
|
著者 |
Murata K, Hayashibara T, Sugahara K, Uemura A, Yamaguchi T, Harasawa H, Hasegawa H, Tsuruda K, Okazaki T, Koji T, Miyanishi T, Yamada Y, Kamihira S.
|
タイトル |
A novel alternative splicing isoform of human T-cell leukemia virus type 1 bZIP factor (HBZ-SI) targets distinct subnuclear localization.
|
ジャーナル |
J Virol
|
Abstract |
Adult T-cell leukemia (ATL) is associated with prior infection with human T-cell leukemia virus type 1 (HTLV-1); however, the mechanism by which HTLV-1 causes adult T-cell leukemia has not been fully elucidated. Recently, a functional basic leucine zipper (bZIP) protein coded in the minus strand of HTLV-1 genome (HBZ) was identified. We report here a novel isoform of the HTLV-1 bZIP factor (HBZ), HBZ-SI, identified by means of reverse transcription-PCR (RT-PCR) in conjunction with 5' and 3' rapid amplification of cDNA ends (RACE). HBZ-SI is a 206-amino-acid-long protein and is generated by alternative splicing between part of the HBZ gene and a novel exon located in the 3' long terminal repeat of the HTLV-1 genome. Consequently, these isoforms share >95% amino acid sequence identity, and differ only at their N termini, indicating that HBZ-SI is also a functional protein. Duplex RT-PCR and real-time quantitative RT-PCR analyses showed that the mRNAs of these isoforms were expressed at equivalent levels in all ATL cell samples examined. Nonetheless, we found by Western blotting that the HBZ-SI protein was preferentially expressed in some ATL cell lines examined. A key finding was obtained from the subcellular localization analyses of these isoforms. Despite their high sequence similarity, each isoform was targeted to distinguishable subnuclear structures. These data show the presence of a novel isoform of HBZ in ATL cells, and in addition, shed new light on the possibility that each isoform may play a unique role in distinct regions in the cell nucleus.
|
巻・号 |
80(5)
|
ページ |
2495-505
|
公開日 |
2006-3-1
|
DOI |
10.1128/JVI.80.5.2495-2505.2006
|
PII |
80/5/2495
|
PMID |
16474156
|
PMC |
PMC1395368
|
MeSH |
Alternative Splicing*
Amino Acid Sequence
Artificial Gene Fusion
Base Sequence
Basic-Leucine Zipper Transcription Factors / chemistry
Basic-Leucine Zipper Transcription Factors / genetics*
Basic-Leucine Zipper Transcription Factors / metabolism*
Biological Transport
Blotting, Western
Cell Line, Tumor
Cell Nucleus / metabolism*
Green Fluorescent Proteins / analysis
Green Fluorescent Proteins / genetics
Human T-lymphotropic virus 1 / genetics*
Humans
Microscopy, Fluorescence
Molecular Sequence Data
Protein Isoforms / chemistry
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA Processing, Post-Transcriptional*
RNA, Messenger / metabolism*
RNA, Viral / metabolism*
Retroviridae Proteins
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Viral Proteins / chemistry
Viral Proteins / genetics*
Viral Proteins / metabolism*
|
IF |
4.501
|
引用数 |
85
|
WOS 分野
|
VIROLOGY
|
リソース情報 |
ヒト・動物細胞 |
COS-7(RCB0539) |