| RRC ID |
42584
|
| Author |
Hashimoto M, Morimoto M, Saimoto H, Shigemasa Y, Sato T.
|
| Title |
Lactosylated chitosan for DNA delivery into hepatocytes: the effect of lactosylation on the physicochemical properties and intracellular trafficking of pDNA/chitosan complexes.
|
| Journal |
Bioconjug Chem
|
| Abstract |
Chitosan is a useful nonviral vector for gene delivery. To make a pDNA/chitosan complex specific to hepatocytes, lactose-modified chitosan (lac-chitosan) was synthesized. When the percentage of lactose residues substituted was 8%, lac-chitosan showed excellent DNA-binding ability, good protection of DNA from nuclease, and the suppression of self-aggregation and serum-induced aggregation. Although the cellular uptake efficiency of the pDNA/lac-chitosan complex was almost the same as that of the pDNA/chitosan complex, the cell transfection efficiency of the former was greater for HepG2 cells having asialoglycoprotein receptors. Inhibitor of endocytosis such as bafilomycin A1 and nocodazole significantly reduced the transfection efficiency of the pDNA/lac-chitosan complex. Observations with a confocal laser scanning microscope indicated that the pDNA/lac-chitosan complexes traversed endocytic compartments more rapidly than the pDNA/chitosan complex. Furthermore, the pDNA/lac-chitosan complexes were delivered to the late endosome and have the advantage of delivering DNA to the perinuclear region.
|
| Volume |
17(2)
|
| Pages |
309-16
|
| Published |
2006-1-1
|
| DOI |
10.1021/bc050228h
|
| PMID |
16536460
|
| MeSH |
Animals
Biocompatible Materials* / chemistry
Biocompatible Materials* / metabolism
Biological Transport
Carbohydrate Conformation
Carbohydrate Sequence
Cell Line
Chitosan* / chemistry
Chitosan* / metabolism
DNA / metabolism*
Deoxyribonuclease I / metabolism
Hepatocytes / metabolism*
Humans
Lactose / chemistry*
Lactose / metabolism
Macromolecular Substances
Molecular Sequence Data
Molecular Structure
Transfection
|
| IF |
4.031
|
| Times Cited |
104
|
|
WOS Category
|
CHEMISTRY, ORGANIC
BIOCHEMICAL RESEARCH METHODS
CHEMISTRY, MULTIDISCIPLINARY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
COS-7(RCB0539)
Hep G2 |
