RRC ID 42591
Author Murakami Y, Shoji M, Hirata A, Tanaka S, Hanazawa S, Yokoe I, Fujisawa S.
Title An ortho dimer of butylated hydroxyanisole inhibits nuclear factor kappa B activation and gene expression of inflammatory cytokines in macrophages stimulated by Porphyromonas gingivalis fimbriae.
Journal Arch Biochem Biophys
Abstract Butylated hydroxyanisole, BHA, is widely used as a potent antioxidant, but its adverse effects such as carcinogenesis and proinflammatory activity have been reported, which are possibly due to the prooxidant property of this compound. We recently demonstrated that the dimer of 2-methoxyphenols exhibits cyclooxygenase-2 inhibition, because of lessening of its prooxidant property caused by the dimerization. In the present study, toward our goal of developing a chemopreventive agent for chronic periodontal diseases, we examined whether 2-BHA (2-tert-butyl-4-methoxyphenol) and its synthetic ortho dimer, bis-BHA (3,3'-di-tert-butyl-5,5'-dimethoxy-1,1'-biphenyl-2,2'-diol) could inhibit the Porphyromonas gingivalis fimbria-stimulated inflammatory reaction. The fimbria-induced expression of interleukin-1beta and neutrophil chemoattractant KC genes in RAW264.7 murine macrophages was strongly inhibited by bis-BHA. In contrast, 2-BHA showed only slight inhibition. bis-BHA also significantly inhibited the fimbria-stimulated phosphorylation-dependent degradation of the alpha inhibitor of nuclear factor-kappaB and the transcriptional activity of this factor in the cells. These findings suggest that bis-BHA possesses anti-inflammatory activity against chronic periodontal diseases.
Volume 449(1-2)
Pages 171-7
Published 2006-5-15
DOI 10.1016/j.abb.2006.02.005
PII S0003-9861(06)00058-0
PMID 16546114
MeSH Animals Anti-Inflammatory Agents / administration & dosage Bacteroidaceae Infections / immunology Bacteroidaceae Infections / prevention & control Butylated Hydroxyanisole / administration & dosage* Butylated Hydroxyanisole / chemistry Cell Line Cytokines / immunology* Dimerization Dose-Response Relationship, Drug Fimbriae, Bacterial / immunology Gene Expression Regulation / immunology Isomerism Macrophage Activation / drug effects Macrophage Activation / immunology* Macrophages / drug effects Macrophages / immunology* Mice NF-kappa B / antagonists & inhibitors* NF-kappa B / immunology* Porphyromonas gingivalis / immunology*
IF 3.391
Times Cited 10
Human and Animal Cells RAW 264(RCB0535)