RRC ID 42605
Author Chen L, Fink T, Ebbesen P, Zachar V.
Title Optimized chondrogenesis of ATCD5 cells through sequential regulation of oxygen conditions.
Journal Tissue Eng
Abstract The objective of this study was to determine the optimal oxygen conditions for chondrogenesis of ATDC5 mouse embryonic stem cells. Chondrogenesis was induced by addition of insulin and the cells were then cultured at different oxygen concentrations ranging from 1 to21%. At 2- to 3-day intervals, chondrocyte-specific extracellular matrix (ECM) production was monitored. Furthermore, the transcription of collagen II, an early-phase marker, and collagen X, a marker of hypertrophic conversion, was followed by real-time RT-PCR. Low oxygen concentrations between 1 and 9% inhibited chondrogenic conversion, as evidenced by reduced glycosaminoglycan deposition in the ECM in a manner proportional to the degree of hypoxia. Cells cultured at oxygen concentrations of 12 and 15% underwent a faster and higher degree of early-phase chondrogenesis when compared to control cells cultured at ambient air (21% O2). For the hypertrophic conversion of the ATDC5 cells, all degrees of hypoxia inhibited collagen X expression in a dose-dependent manner. Short-term culturing of the ATDC5 cells for 6 to 8 days at 12% oxygen with subsequent culturing at 21% for the remainder of the experiment resulted in maximal production of major ECM components, including collagen II and glycosaminoglycans. It is thus possible to modify in vitro chondrogenesis through modulation of the gas-phase composition.
Volume 12(3)
Pages 559-67
Published 2006-3-1
DOI 10.1089/ten.2006.12.559
PMID 16579689
MeSH Animals Cell Hypoxia Cell Line Chondrogenesis / drug effects Chondrogenesis / physiology* Collagen Type II / genetics Collagen Type X / genetics Extracellular Matrix / drug effects Extracellular Matrix / metabolism Gene Expression Regulation / drug effects Glycosaminoglycans / biosynthesis Insulin / pharmacology Mice Oxygen / administration & dosage Oxygen / metabolism* RNA / genetics RNA / metabolism Stem Cells / cytology Stem Cells / drug effects Stem Cells / metabolism Tissue Engineering
IF 3.508
Times Cited 19
Human and Animal Cells ATDC5(RCB0565)