論文 - 詳細
| RRC ID | 42614 |
|---|---|
| 著者 | Inoh K, Muramatsu H, Torii S, Ikematsu S, Oda M, Kumai H, Sakuma S, Inui T, Kimura T, Muramatsu T. |
| タイトル | Doxorubicin-conjugated anti-midkine monoclonal antibody as a potential anti-tumor drug. |
| ジャーナル | Jpn J Clin Oncol |
| Abstract |
BACKGROUND:Midkine is a heparin-binding growth factor preferentially expressed in tumor cells. The present study was performed to utilize anti-midkine antibody for tumor therapy. METHODS:A monoclonal antibody to midkine was raised by immunizing mice deficient in the midkine gene. The binding site of the antibody was studied by using N-terminal half and C-terminal half of midkine, both of which were chemically synthesized. Doxorubicin (DOX)-conjugate of the antibody was produced by chemical conjugation. The effects of the antibody and the conjugate on cell growth were examined using a midkine-secreting tumor cell, i.e. human hepatocellular carcinoma cell (HepG2). RESULTS:The monoclonal antibody bound to the N-terminal half of midkine. The antibody did not inhibit the growth of HepG2 cells probably because the active domain of midkine is in the C-terminal half. We produced the antibody conjugated with DOX with the hope that the conjugate would be internalized accompanied with midkine. Indeed, the antibody-DOX conjugate significantly inhibited the growth of HepG2 cells compared with DOX-conjugated control IgG. CONCLUSION:The result raises the possibility of using anti-midkine antibody conjugated with DOX for cancer therapy. |
| 巻・号 | 36(4) |
| ページ | 207-11 |
| 公開日 | 2006-4-1 |
| DOI | 10.1093/jjco/hyl004 |
| PII | hyl004 |
| PMID | 16611663 |
| MeSH | Antibiotics, Antineoplastic / pharmacology* Antibiotics, Antineoplastic / therapeutic use Antibodies, Monoclonal / pharmacology Antibodies, Monoclonal / therapeutic use Carcinoma, Hepatocellular / pathology* Cell Survival / drug effects Cytokines / immunology* Doxorubicin / pharmacology* Doxorubicin / therapeutic use Humans Immunotoxins / pharmacology* Immunotoxins / therapeutic use Liver Neoplasms / pathology* Midkine Tumor Cells, Cultured |
| IF | 1.914 |
| 引用数 | 19 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |