論文 - 詳細
| RRC ID | 42619 |
|---|---|
| 著者 | Sekikawa T, Iwase S, Saito S, Arakawa Y, Agawa M, Horiguchi-Yamada J, Yamada H. |
| タイトル | JAS-R, a new megakaryo-erythroid leukemic cell line that secretes erythropoietin. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:The processes of leukemogenesis and differentiation of the megakaryo-erythroid lineage remain poorly understood. Leukemic cell lines derived from megakaryocytic leukemia are valuable reagents for studies on these events. MATERIALS AND METHODS:A new cell line, JAS-R, was established from a 64-year-old patient with acute megakaryocytic leukemia (AML M7). Its characteristics were studied by morphological, immunophenotypic and molecular biological analysis. RESULTS:Immunophenotyping showed that the JAS-R cells were positive for CD33, CD41 and CD61, as well as moderately to weakly positive for CD4, CD7, CD13 and glycophorin A. Chromosomal analysis revealed a composite karyotype, but no major translocation abnormalities were observed. Electron microscopy disclosed that the JAS-R cells had numerous surface blebs and some cells also had alpha-granules and demarcation membranes. The mRNAs of 4 major proteins (platelet factor 4, beta-thromboglobulin, selectin-P and thrombospondin 1) found in alpha-granules were all expressed by the JAS-R cells. In particular, expression of platelet factor 4 was high. To further characterize JAS-R cells, comparison with 4 other megakaryo-etythroid cell lines (CMK, MEG-01, K562 and KU812) was done by gene expression profiling using an oligo-DNA microarray. The results showed that JAS-R was a distinctive cell line. It was noteworthy that the JAS-R cells secreted erythropoietin and expressed erythropoietin receptor. A neutralizing antibody for erythropoietin partly inhibited the proliferation of the cells. CONCLUSION:JAS-R may be a useful cell line for investigating the differentiation and leukemogenesis of megakaryo-erythroid cells and for studying the influence of erythropoietin on these cells. |
| 巻・号 | 26(2A) |
| ページ | 843-50 |
| 公開日 | 2006-1-1 |
| PMID | 16619478 |
| MeSH | Cell Line, Tumor / pathology* Erythropoietin / metabolism* Female Humans Immunophenotyping K562 Cells Karyotyping Leukemia, Erythroblastic, Acute / genetics Leukemia, Erythroblastic, Acute / pathology* Leukemia, Megakaryoblastic, Acute / genetics Leukemia, Megakaryoblastic, Acute / pathology* Middle Aged |
| IF | 1.994 |
| 引用数 | 4 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | K562 |