| RRC ID |
42621
|
| 著者 |
Calvopina M, Barroso PA, Marco JD, Korenaga M, Cooper PJ, Nonaka S, Hashiguchi Y.
|
| タイトル |
Efficacy of vaccination with a combination of Leishmania amastigote antigens and the lipid A-analogue ONO-4007 for immunoprophylaxis and immunotherapy against Leishmania amazonensis infection in a murine model of New World cutaneous leishmaniasis.
|
| ジャーナル |
Vaccine
|
| Abstract |
Activation of innate immunity using adjuvants that activate Toll-like receptor 4 pathways have great potential for improving the protection induced by parasite vaccines. We investigated protective and therapeutic effects of a vaccine against leishmaniasis containing a combination of an adjuvant synthetic lipid A-analogue, ONO-4007 and Leishmania amazonensis antigens. ONO-4007 was co-injected with soluble and membrane-enriched L. amazonensis-amastigote antigens into BALB/c mice that had either already been infected with 1 x 10(6) L. amazonensis promastigotes (immunotherapy study) or before challenge with the same infectious dose (immunoprophylaxis study). Sixty percent of mice vaccinated before infectious challenge controlled their Leishmania infections - defined by the absence of footpad-swelling and negative Leishmania cultures - compared to 0% of controls, and 40% of mice vaccinated after infection resolved their infections compared to 0% of controls. Protective immunity in both immunoprophylaxis and immunotherapy models was associated with increased protein production of IL-12 and IFN-gamma. These data suggest that vaccination with a combination of ONO-4007 and amastigote antigens of L. amazonensis may be useful for the prevention and treatment of leishmaniasis, and that the protective immunity induced is associated with the production of type-1 cytokines.
|
| 巻・号 |
24(27-28)
|
| ページ |
5645-52
|
| 公開日 |
2006-7-7
|
| DOI |
10.1016/j.vaccine.2006.03.023
|
| PII |
S0264-410X(06)00282-9
|
| PMID |
16621179
|
| MeSH |
Animals
Antigens, Protozoan / immunology*
Disease Models, Animal
Female
Interferon-gamma / immunology
Interleukin-12 / immunology
Leishmania / immunology*
Leishmaniasis, Cutaneous / immunology
Leishmaniasis, Cutaneous / prevention & control
Leishmaniasis, Cutaneous / therapy*
Lipid A / administration & dosage
Lipid A / analogs & derivatives*
Lipid A / immunology
Mice
Mice, Inbred BALB C
Protozoan Vaccines / administration & dosage*
Protozoan Vaccines / immunology
|
| IF |
3.143
|
| 引用数 |
16
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
J774.1(RCB0434) |
