RRC ID 42644
Author Terakawa M, Tomimori Y, Goto M, Fukuda Y.
Title Mast cell chymase induces expression of chemokines for neutrophils in eosinophilic EoL-1 cells and mouse peritonitis eosinophils.
Journal Eur J Pharmacol
Abstract Human chymase induced release of interleukin-8 (IL-8) in human EoL-1 cells that had been differentiated into eosinophil-like cells with butyric acid. The chymase-induced IL-8 production was specific in that other cytokines/chemokines examined were not induced. Human chymase also increased mRNA for IL-8 in the differentiated EoL-1 cells, showing involvement of mRNA synthesis. The chymase-induced IL-8 release was inhibited by pertussis toxin as well as U0126 (an inhibitor for extracellular signal-regulated kinase pathway) and SB203580 (p38 inhibitor), suggesting that the chymase-induced IL-8 production is mediated by G protein-coupled receptor and mitogen-activated protein kinases. Mouse mast cell protease-4 (mMCP-4), a mouse chymase, induced macrophage-inflammatory protein-2 (MIP-2), a mouse homologue for IL-8, in mouse eosinophils in vitro. Intradermal injection of mMCP-4 not only induced skin edema but increased MIP-2 content and neutrophil number at the injection site. Taken together, our findings demonstrate that mast cell chymase may contribute to the interaction between eosinophils and neutrophils by inducing IL-8/MIP-2 in eosinophils at allergic inflamed sites.
Volume 538(1-3)
Pages 175-81
Published 2006-5-24
DOI 10.1016/j.ejphar.2006.03.053
PII S0014-2999(06)00344-X
PMID 16690053
MeSH Animals Butadienes / pharmacology Cell Line, Tumor Chemokine CXCL2 Chemokines / genetics Chemokines / metabolism* Chymases Cytokines / genetics Cytokines / metabolism Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Eosinophils / drug effects* Eosinophils / metabolism Eosinophils / pathology Gene Expression / drug effects Humans Imidazoles / pharmacology Interleukin-8 / genetics Interleukin-8 / metabolism Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinases / antagonists & inhibitors Neutrophils / drug effects* Neutrophils / metabolism Neutrophils / pathology Nitriles / pharmacology Peritonitis / genetics Peritonitis / metabolism Peritonitis / pathology* Pertussis Toxin / pharmacology Pyridines / pharmacology Quinazolines / pharmacology RNA, Messenger / genetics RNA, Messenger / metabolism Serine Endopeptidases / pharmacology*
IF 3.17
Times Cited 28
Human and Animal Cells