RRC ID 42645
Author Yasutake K, Binh ND, Kagoshima K, Uemori A, Ohgushi A, Maeda M, Mizuki E, Yu YM, Ohba M.
Title Occurrence of parasporin-producing Bacillus thuringiensis in Vietnam.
Journal Can. J. Microbiol.
Abstract A total of 63 Bacillus thuringiensis isolates were recovered from urban soils of Hanoi, Vietnam. Of these, 34 were identified to 12 H serogroups. None of the isolates showed larvicidal activities against three lepidopterous insects. Three isolates belonging to the two serovars, colmeri (H21) and konkukian (H34), were highly toxic to larvae of the mosquito Aedes aegypti. Parasporal inclusion proteins of four isolates exhibited cytocidal activities against HeLa cells. Immunologically, proteins of four isolates were closely related to parasporin-1 (Cry31Aa), a parasporal protein that preferentially kills human cancer cells. Haemolytic activities were associated with parasporal proteins of the three mosquitocidal isolates but not with those of the four cancer-cell-killing isolates. PCR experiments and nucleotide sequence analysis revealed that the genes of four anti-cancer isolates are closely related to the gene parasporin-1 (cry31Aa) but are dissimilar to those of the three other existing parasporins. Our results suggest that the soil of northern Vietnam is a good reservoir of parasporin-producing B. thuringiensis.
Volume 52(4)
Pages 365-72
Published 2006-4
DOI 10.1139/w05-134
PII w05-134
PMID 16699587
MeSH Animals Antigens, Bacterial / metabolism Bacillus thuringiensis / classification Bacillus thuringiensis / genetics Bacillus thuringiensis / metabolism* Bacterial Proteins / biosynthesis Bacterial Proteins / genetics Bacterial Proteins / toxicity Cell Survival / drug effects Endotoxins / biosynthesis* Endotoxins / genetics Endotoxins / toxicity HeLa Cells Hemolysis / drug effects Humans Inclusion Bodies / chemistry Insecta / drug effects Microscopy, Phase-Contrast Molecular Sequence Data Polymerase Chain Reaction Serotyping Sheep Soil Microbiology Vietnam
IF 1.243
Times Cited 18
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY IMMUNOLOGY MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells