RRC ID 42647
著者 Nakamura K, Miyagi K, Koguchi Y, Kinjo Y, Uezu K, Kinjo T, Akamine M, Fujita J, Kawamura I, Mitsuyama M, Adachi Y, Ohno N, Takeda K, Akira S, Miyazato A, Kaku M, Kawakami K.
タイトル Limited contribution of Toll-like receptor 2 and 4 to the host response to a fungal infectious pathogen, Cryptococcus neoformans.
ジャーナル FEMS Immunol Med Microbiol
Abstract The present study was designed to elucidate the role of Toll-like receptor (TLR) 2 and TLR4 in the host response to Cryptococcus neoformans. Both TLR2 knockout (KO) and TLR4KO mice produced interleukin-1beta (IL-1beta), IL-6, IL-12p40 and tumor necrosis factor-alpha (TNF-alpha) in sera and cleared this fungal pathogen from infected lungs at a comparable level to control littermate (LM) mice. Synthesis of these cytokines was not significantly different in the lungs of these KO mice and LM mice, although IL-1beta, IL-6 and IL-12p40 tended to be lower in TLR2KO, but not TLR4KO, mice than in controls. In addition, there was no significant reduction detected in the synthesis of IL-12 and TNF-alpha by bone marrow-derived dendritic cells from TLR2KO and TLR4KO mice upon stimulation with live yeast cells. Finally, HEK293 cells expressing either TLR2/dectin-1 or TLR4/MD2/CD14 did not respond to C. neoformans in the activation of nuclear factor kappa B (NFkappaB) detected by a luciferase assay. Our results suggest that TLR2 and TLR4 do not or only marginally contribute to the host and cellular response to this pathogen.
巻・号 47(1)
ページ 148-54
公開日 2006-6-1
DOI 10.1111/j.1574-695X.2006.00078.x
PII FIM078
PMID 16706798
MeSH Animals Cryptococcosis / immunology* Cryptococcus neoformans / immunology* Dendritic Cells / immunology Female Interleukin-1 / biosynthesis Interleukin-1 / immunology Interleukin-12 / biosynthesis Interleukin-12 / immunology Interleukin-12 Subunit p40 Interleukin-6 / biosynthesis Interleukin-6 / immunology Male Mice Mice, Inbred C57BL Mice, Knockout Protein Subunits / biosynthesis Protein Subunits / immunology Toll-Like Receptor 2 / deficiency Toll-Like Receptor 2 / genetics Toll-Like Receptor 2 / immunology* Toll-Like Receptor 4 / deficiency Toll-Like Receptor 4 / genetics Toll-Like Receptor 4 / immunology* Transfection Tumor Necrosis Factor-alpha / biosynthesis Tumor Necrosis Factor-alpha / immunology
引用数 58
WOS 分野 INFECTIOUS DISEASES MICROBIOLOGY IMMUNOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)