| RRC ID |
42648
|
| Author |
Suzuki T, Koyama Y, Hayakawa S, Munakata H, Isemura M.
|
| Title |
1,25-Dihydroxyvitamin D3 suppresses exportin expression in human promyelocytic leukemia HL-60 cells.
|
| Journal |
Biomed Res
|
| Abstract |
The active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3 (DVD), is a potent inducer of cell differentiation and inhibits proliferation of cells such as human promyelocytic leukemia HL-60 cells. In the present study, we examined the effects of DVD on the expression of exportin-1 and exportin-t, which play essential roles in the transport of mRNA and tRNA, respectively. The results of reverse transcription-polymerase chain reaction and quantitative real-time polymerase chain reaction indicated that DVD down-regulated the gene expression of these exportins. Western blotting revealed the decreased production of these proteins in DVD-treated cells. Thus, the present findings of decreased expression of exportin-1 and exportin-t induced by DVD can be correlated to inhibition of the proliferation of HL-60 cells.
|
| Volume |
27(2)
|
| Pages |
89-92
|
| Published |
2006-4-1
|
| DOI |
10.2220/biomedres.27.89
|
| PII |
JST.JSTAGE/biomedres/27.89
|
| PMID |
16707848
|
| MeSH |
Cell Proliferation
Gene Expression Regulation, Leukemic
HL-60 Cells
Humans
Karyopherins / genetics
Karyopherins / metabolism*
Leukemia, Promyelocytic, Acute / metabolism*
Nucleocytoplasmic Transport Proteins / genetics
Nucleocytoplasmic Transport Proteins / metabolism*
RNA, Messenger / metabolism
RNA, Transfer / metabolism
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Cytoplasmic and Nuclear / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Vitamin D / analogs & derivatives*
Vitamin D / metabolism
|
| IF |
1.429
|
| Times Cited |
12
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
|
| Resource |
| Human and Animal Cells |
HL-60 |
