| RRC ID |
42655
|
| 著者 |
Katsumata N, Horikawa R, Tanaka T.
|
| タイトル |
Replacement of alanine with asparagic acid at position 203 in human steroidogenic acute regulatory protein impairs the ability to enhance steroidogenesis in vitro.
|
| ジャーナル |
Endocr J
|
| Abstract |
Steroidogenic acute regulatory protein (StAR) is a 30-kDa phosphorylated protein that rapidly appears in mitochondria of steroidogenic cells following tropic stimulation, and is required in the acute regulation of steroidogenesis. It was reported that mutations in the STAR gene encoding StAR cause congenital lipoid adrenal hyperplasia (CLAH), an autosomal recessive disorder characterized by impaired synthesis of all adrenal and gonadal steroid hormones. We previously reported a D203A polymorphism in the STAR gene in Japanese patients with CLAH as well as in normal Japanese subjects. In the present study, we analyzed the ability of the A203 StAR and D203 StAR to stimulate steroidogenesis using the in vitro functional expression system. The A203 StAR caused a twelve-fold increase in pregnenolone secretion over COS-1 cells transfected with an NH2-cholesterol side-chain cleavage enzyme (P450scc)-adrenodoxin reductase-adrenodoxin-COOH fusion protein expressing plasmid (F2) and an empty vector, whereas the D203 StAR increased pregnenolone production no more than threefold. Western blot analysis detected mainly two species of StAR consisting of the 37-kDa precursor and the 30-kDa mature form. Together, these results indicate that the alanine at position 203 in human StAR is functionally important and that the D203 StAR is extremely unlikely to be a polymorphism.
|
| 巻・号 |
53(3)
|
| ページ |
427-31
|
| 公開日 |
2006-6-1
|
| DOI |
10.1507/endocrj.k05-174
|
| PII |
JST.JSTAGE/endocrj/K05-174
|
| PMID |
16723808
|
| MeSH |
Alanine / genetics*
Amino Acid Substitution / genetics*
Animals
Asparagine / genetics*
COS Cells
Chlorocebus aethiops
Humans
Phosphoproteins / genetics*
Phosphoproteins / metabolism*
Pregnenolone / biosynthesis
Steroids / biosynthesis*
Transfection
|
| IF |
1.952
|
| 引用数 |
6
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
COS-1(RCB0143) |
