| RRC ID |
42656
|
| Author |
Islam S, Hassan F, Tumurkhuu G, Ito H, Koide N, Mori I, Yoshida T, Yokochi T.
|
| Title |
5-Fluorouracil prevents lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells by inhibiting Akt-dependent nuclear factor-kappaB activation.
|
| Journal |
Cancer Chemother Pharmacol
|
| Abstract |
The effect of 5-fluorouracil (5-FU) on the production of nitric oxide (NO) in macrophages was examined by using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. 5-FU at non-toxic concentrations significantly inhibited NO production in LPS-stimulated RAW 264.7 cells. The inhibition by 5-FU was mediated by attenuated expression of an inducible NO synthase protein and mRNA. 5-FU inhibited the activation of nuclear factor (NF)-kappaB and the subsequent nuclear translocation. Furthermore, 5-FU inhibited the phosphorylation of Akt, an upstream molecule of NF-kappaB signaling. 5-FU did not affect a series of mitogen-activated protein kinases. Therefore, 5-FU was suggested to inhibit the LPS-induced NO production in activated macrophages through preventing Akt-dependent NF-kappaB activation.
|
| Volume |
59(2)
|
| Pages |
227-33
|
| Published |
2007-2-1
|
| DOI |
10.1007/s00280-006-0261-2
|
| PMID |
16724238
|
| MeSH |
Active Transport, Cell Nucleus / drug effects
Animals
Antimetabolites, Antineoplastic / pharmacology
Cell Line
Cell Nucleus / drug effects
Cell Nucleus / metabolism
Cell Survival / drug effects
Dose-Response Relationship, Drug
Fluorouracil / pharmacology*
Lipopolysaccharides / pharmacology*
Luciferases / genetics
Luciferases / metabolism
Macrophages / cytology
Macrophages / drug effects*
Macrophages / metabolism
Mitogen-Activated Protein Kinases / metabolism
NF-kappa B / antagonists & inhibitors*
NF-kappa B / metabolism
Nitric Oxide / biosynthesis*
Nitric Oxide Synthase Type II / antagonists & inhibitors
Nitric Oxide Synthase Type II / genetics
Nitric Oxide Synthase Type II / metabolism
Proto-Oncogene Proteins c-akt / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
|
| IF |
2.967
|
| Times Cited |
10
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
|
