Reference - Detail
| RRC ID | 42657 |
|---|---|
| Author | Takahashi E, Inanami O, Ohta T, Matsuda A, Kuwabara M. |
| Title | Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines. |
| Journal | Leuk Res |
| Abstract |
To clarify the role of lipid rafts in 2-chloro-2'-deoxyadenosine (2CdA; Cladribine)-induced apoptosis, the effects of disruption of lipid rafts by methyl-beta-cyclodextrin (MbetaCD) and filipin on 2CdA-induced apoptosis were investigated in four human acute lymphoblastic leukemia (ALL) cell lines comprised of T cells (MOLT-4, Jurkat) and B cells (NALM, BALL-1). The disruption of lipid rafts significantly inhibited 2CdA-induced apoptosis, indicating the crucial role of lipid rafts in the induction of apoptosis in leukemia cells. These reagents significantly inhibited 2CdA-induced elevation of the intracellular calcium concentration ([Ca(2+)](i)) in MOLT-4 cells, and 2CdA-induced apoptosis was partly inhibited by the Ca(2+) chelators BAPTA-AM and EGTA, and the L-type Ca(2+) channel blocker nifedipine. On the other hand, they had no effects on the cellular uptake of 2CdA. These results indicated that lipid rafts partly contributed to 2CdA-induced apoptosis by regulating Ca(2+) influx via the plasma membrane. |
| Volume | 30(12) |
| Pages | 1555-61 |
| Published | 2006-12-1 |
| DOI | 10.1016/j.leukres.2006.04.008 |
| PII | S0145-2126(06)00147-0 |
| PMID | 16730061 |
| MeSH | Apoptosis / drug effects* Calcium / metabolism Cell Line, Tumor Chelating Agents / pharmacology Cladribine / antagonists & inhibitors Cladribine / pharmacology* Drug Screening Assays, Antitumor Egtazic Acid / analogs & derivatives Egtazic Acid / pharmacology Filipin / pharmacology Humans Membrane Microdomains / drug effects Membrane Microdomains / metabolism* Nifedipine / pharmacology Precursor Cell Lymphoblastic Leukemia-Lymphoma / drug therapy Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism* Time Factors beta-Cyclodextrins / pharmacology |
| IF | 2.214 |
| Times Cited | 11 |
| WOS Category | HEMATOLOGY ONCOLOGY |
| Resource | |
| Human and Animal Cells | MOLT-4(RCB0206) |