RRC ID 42662
Author Hori M, Sawai H, Tsuji Y, Okamura H, Koyama K.
Title Bone morphogenetic protein-2 counterregulates interleukin-18 mRNA and protein in MC3T3-E1 mouse osteoblastic cells.
Journal Connect Tissue Res
Abstract Fibroblast growth factors-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) are two of the main factors that regulate differentiation of osteoblasts. Interleukin-18 (IL-18), originally cloned as an interferon gamma-inducing factor, has been reported to inhibit maturation of osteoclasts by upregulation of osteoprotegerin secreted from osteoblasts. Little is known about the functional relationship between IL-18 and the two growth factors in osteoblast differentiation. To better understand this relationship, we analyzed the effect of BMP-2 and FGF-2 on the mRNA expression levels of IL-18, as well as IL-1alpha and IL-6, in MC3T3-E1 mouse osteoblastic cells. Following this, the effects of BMP-2 on the expression of IL-18 protein and caspase-1 protein were analyzed by immunofluorescence staining. Real-time PCR and immunofluorescence staining analysis showed that FGF-2 had no effect on the expression of IL-18 mRNA and protein, but while BMP-2 reduced IL-18 mRNA levels, increased immunostaining of both IL-18 protein and caspase-1 protein was detected in BMP-2-treated MC3T3-E1 cells. Although the significance and mechanisms of this counterregulation of IL-18 mRNA and protein were not determined in this study, the increase of IL-18 protein suggested that BMP-2 may induce an active form of IL-18.
Volume 47(3)
Pages 124-32
Published 2006-1-1
DOI 10.1080/03008200600685350
PII VN7316QR30358282
PMID 16753805
MeSH Alkaline Phosphatase / metabolism Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / pharmacology* Caspase 1 / metabolism Cell Line Fibroblast Growth Factor 2 / pharmacology Gene Expression Regulation / drug effects* Interleukin-1 / genetics Interleukin-1 / metabolism Interleukin-18 / genetics* Interleukin-18 / metabolism Interleukin-6 / genetics Interleukin-6 / metabolism Mice Osteoblasts / drug effects* Osteoblasts / metabolism RNA, Messenger / metabolism* Recombinant Proteins / pharmacology Time Factors Transforming Growth Factor beta / pharmacology*
IF 2.795
Times Cited 9
Human and Animal Cells MC3T3-E1(RCB1126)