RRC ID 42664
著者 Fujita Y, Hara Y, Nezu Y, Schulz KS, Tagawa M.
タイトル Proinflammatory cytokine activities, matrix metalloproteinase-3 activity, and sulfated glycosaminoglycan content in synovial fluid of dogs with naturally acquired cranial cruciate ligament rupture.
ジャーナル Vet Surg
Abstract OBJECTIVE:To measure and compare activities of interleukin-1beta (IL-1beta), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and matrix metalloproteinase-3 (MMP-3); as well as sulfated glycosaminoglycan (S-GAG) content in synovial fluid from dogs with cranial cruciate ligament rupture (CCLR) and dogs with clinically normal stifles. To determine whether correlations exist between demographic and disease-related variables and these synovial markers.
STUDY DESIGN:Prospective clinical study.
ANIMALS:Dogs with CCLR (n=23) and Beagles with normal stifle joints (n=21).
METHODS:Synovial fluid activities of proinflammatory cytokines (IL-1beta, IL-6, and TNF-alpha) were determined by bioassay. MMP-3 activity was measured using fluorogenic substrate. S-GAG contents were determined by dimethylmethylene blue dye-binding assay. Mann-Whitney U-test was used to compare results from CCLR joints with normal controls. Spearman's rank correlation test was used to evaluate associations between demographic and disease-related markers and synovial markers.
RESULTS:Mean values for synovial markers were significantly higher in CCLR joints compared with controls. IL-1beta and MMP-3 were positively correlated with lameness duration.
CONCLUSIONS:Activities of proinflammatory cytokines, MMP-3 activity and S-GAG contents were significantly elevated in synovial fluid from canine stifle joints with naturally acquired CCLR. These results indicate that there is joint inflammation and increased release of GAGs into synovial fluid, suggesting that these inflammatory changes are associated with depletion of proteoglycan from articular cartilage.
CLINICAL RELEVANCE:Medical and surgical treatments designed to decrease joint inflammation and breakdown of proteoglycans may be of value in the management of CCLR in the dog.
巻・号 35(4)
ページ 369-76
公開日 2006-6-1
DOI 10.1111/j.1532-950X.2006.00159.x
PII VSU00159
PMID 16756618
MeSH Animals Anterior Cruciate Ligament Injuries* Biomarkers / metabolism Case-Control Studies Cytokines / metabolism Dogs / injuries* Dogs / surgery Female Glycosaminoglycans / metabolism Injury Severity Score Interleukin-1 / metabolism Interleukin-6 / metabolism Knee Injuries / diagnostic imaging Knee Injuries / metabolism Knee Injuries / surgery Knee Injuries / veterinary* Male Matrix Metalloproteinase 3 / metabolism Prospective Studies Radiography Synovial Fluid / metabolism* Tumor Necrosis Factor-alpha / metabolism
IF 1.255
引用数 35
WOS 分野 VETERINARY SCIENCES
リソース情報
ヒト・動物細胞 7-TD-1(RCB1190)