RRC ID 42668
Author Kawakami Y, Seoka M, Miyashita S, Kumai H, Ohta H.
Title Characterization of transthyretin in the Pacific bluefin tuna, Thunnus orientalis.
Journal Zool. Sci.
Abstract A cDNA encoding transthyretin was cloned from the Pacific bluefin tuna (Thunnus orientalis). This cDNA contains a complete open reading frame encoding 151 amino acid residues. The deduced amino acid sequence is 81% and 55% identical to the gilthead seabream and common carp forms, respectively, and 33-39% to mammalian, reptilian, and amphibian forms. A 1.0-kb transcript was found in the the liver and ovary; the liver is the main source of this protein. Analysis of triiodo-L-thyronine (T(3)) and L-thyroxine (T(4)) binding demonstrated that both T(3) and T(4) bind to bluefin transthyretin. The binding activity of T(3) for bluefin transthyretin is higher than that of T(4). These results indicate that bluefin transthyretin acts as a transporter of thyroid hormones (THs) in the plasma, and plays an important role in the function of THs in target cells.
Volume 23(5)
Pages 443-8
Published 2006-5
DOI 10.2108/zsj.23.443
PII JST.JSTAGE/zsj/23.443
PMID 16766863
MeSH Amino Acid Sequence Animals Base Sequence DNA, Complementary / analysis* DNA, Complementary / chemistry Female Gene Expression Liver / metabolism* Male Molecular Sequence Data Open Reading Frames Ovary / metabolism Prealbumin / chemistry Prealbumin / genetics* Prealbumin / isolation & purification* RNA, Messenger / biosynthesis Reverse Transcriptase Polymerase Chain Reaction / veterinary Sequence Alignment Sequence Homology, Nucleic Acid Thyroid Hormones / metabolism* Thyroxine / metabolism Triiodothyronine / metabolism Tuna / blood Tuna / metabolism*
IF 0.906
Times Cited 18
Human and Animal Cells