論文 - 詳細
| RRC ID | 42674 |
|---|---|
| 著者 | Kajiwara M, Kuraku S, Kurokawa T, Kato K, Toda S, Hirose H, Takahashi S, Shibata Y, Iguchi T, Matsumoto T, Miyata T, Miura T, Takahashi Y. |
| タイトル | Tissue preferential expression of estrogen receptor gene in the marine snail, Thais clavigera. |
| ジャーナル | Gen Comp Endocrinol |
| Abstract |
Sex steroid hormones have been widely detected in molluscs, and experiments have shown the importance of sex steroids in sex determination, gonadal tissue maturation and gametogenesis. Nevertheless, the signaling pathways of sex steroids in invertebrates have not yet been elucidated. In order to gain insights into the mechanism of sex steroid signaling in molluscs, we have, therefore, tried to isolate molluscan estrogen receptors from the prosobranch mollusc Thais clavigera. Cerebral ganglia of T. clavigera (Mollusca, Gastropoda, Prosobranchia) were subjected to RNA extraction, and degenerate primers for amino acid sequences conserved in vertebrate estrogen receptors were designed. PCR amplification using cerebral RNA and degenerate primers followed by 5'- and 3'-RACE identified the cDNA encoding T. clavigera estrogen receptor 1 (tcER1). The deduced amino acid sequence showed 93% identity in the DNA-binding domain and 72% identity in the ligand binding domain when compared to Aplysia estrogen receptor. Reporter gene assay revealed that tcER1 is constitutively active and unresponsive to estrogen. Quantitative analysis of the tcER1 mRNA level demonstrated the preferential expression in the ovary. Furthermore, cerebral ganglia expressed tcER1 at a high level in the spring followed by subsequent enlargement of the ovary in later seasons. These results suggest importance of tcER1 in the seasonal development of reproductive organs in T. clavigera. |
| 巻・号 | 148(3) |
| ページ | 315-26 |
| 公開日 | 2006-9-15 |
| DOI | 10.1016/j.ygcen.2006.03.016 |
| PII | S0016-6480(06)00104-3 |
| PMID | 16782100 |
| MeSH | Amino Acid Sequence Animals Aplysia / genetics Aplysia / metabolism Base Sequence COS Cells Chlorocebus aethiops Cloning, Molecular DNA, Complementary / isolation & purification Female Gene Expression Genetic Variation Male Molecular Sequence Data Organ Specificity Protein Structure, Tertiary Radioligand Assay Receptors, Estrogen / genetics* Receptors, Estrogen / isolation & purification Receptors, Estrogen / metabolism Receptors, Estrogen / physiology Recombinant Fusion Proteins / metabolism Sequence Homology, Amino Acid Snails / genetics* Snails / metabolism Snails / physiology Transfection |
| IF | 2.428 |
| 引用数 | 87 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-1(RCB0143) |