| RRC ID |
42674
|
| 著者 |
Kajiwara M, Kuraku S, Kurokawa T, Kato K, Toda S, Hirose H, Takahashi S, Shibata Y, Iguchi T, Matsumoto T, Miyata T, Miura T, Takahashi Y.
|
| タイトル |
Tissue preferential expression of estrogen receptor gene in the marine snail, Thais clavigera.
|
| ジャーナル |
Gen Comp Endocrinol
|
| Abstract |
Sex steroid hormones have been widely detected in molluscs, and experiments have shown the importance of sex steroids in sex determination, gonadal tissue maturation and gametogenesis. Nevertheless, the signaling pathways of sex steroids in invertebrates have not yet been elucidated. In order to gain insights into the mechanism of sex steroid signaling in molluscs, we have, therefore, tried to isolate molluscan estrogen receptors from the prosobranch mollusc Thais clavigera. Cerebral ganglia of T. clavigera (Mollusca, Gastropoda, Prosobranchia) were subjected to RNA extraction, and degenerate primers for amino acid sequences conserved in vertebrate estrogen receptors were designed. PCR amplification using cerebral RNA and degenerate primers followed by 5'- and 3'-RACE identified the cDNA encoding T. clavigera estrogen receptor 1 (tcER1). The deduced amino acid sequence showed 93% identity in the DNA-binding domain and 72% identity in the ligand binding domain when compared to Aplysia estrogen receptor. Reporter gene assay revealed that tcER1 is constitutively active and unresponsive to estrogen. Quantitative analysis of the tcER1 mRNA level demonstrated the preferential expression in the ovary. Furthermore, cerebral ganglia expressed tcER1 at a high level in the spring followed by subsequent enlargement of the ovary in later seasons. These results suggest importance of tcER1 in the seasonal development of reproductive organs in T. clavigera.
|
| 巻・号 |
148(3)
|
| ページ |
315-26
|
| 公開日 |
2006-9-15
|
| DOI |
10.1016/j.ygcen.2006.03.016
|
| PII |
S0016-6480(06)00104-3
|
| PMID |
16782100
|
| MeSH |
Amino Acid Sequence
Animals
Aplysia / genetics
Aplysia / metabolism
Base Sequence
COS Cells
Chlorocebus aethiops
Cloning, Molecular
DNA, Complementary / isolation & purification
Female
Gene Expression
Genetic Variation
Male
Molecular Sequence Data
Organ Specificity
Protein Structure, Tertiary
Radioligand Assay
Receptors, Estrogen / genetics*
Receptors, Estrogen / isolation & purification
Receptors, Estrogen / metabolism
Receptors, Estrogen / physiology
Recombinant Fusion Proteins / metabolism
Sequence Homology, Amino Acid
Snails / genetics*
Snails / metabolism
Snails / physiology
Transfection
|
| IF |
2.428
|
| 引用数 |
87
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
COS-1(RCB0143) |
