Reference - Detail
| RRC ID | 42683 |
|---|---|
| Author | Kim HJ, Cho YD, Leem KH, Lee DN, Kim EH, Kim MG, Kim DK, Shin TY, Boo Y, Lee JH, Kim HK. |
| Title | Effects of ephedrae herba on melanogenesis and gene expression profiles using cDNA microarray in B16 melanocytes. |
| Journal | Phytother Res |
| Abstract |
Ephedrae Herba (EH) is obtained by drying the stems of Ephedra sinica Stapf (family Ephedraceae). EH has been used clinically to treat colds and to reduce edema in skin. The effects of EH on melanogenesis were studied in B16 murine melanocytes. The tyrosinase activity and melanin content were measured after incubation with EH. Both reverse transcription-polymerase chain reaction and cDNA microarray analysis were used to study the mechanism of EH action. EH decreased tyrosinase activity and melanin content in a dose-dependent manner. EH decreased microphthalmia-associated transcription factor (MITF) and tyrosinase gene expression. These effects were controlled by MITF-mediated regulation of tyrosinase gene expression. EH also altered the expression of about 100 other genes. These results suggest that EH may be used clinically to treat freckles and liver spots. |
| Volume | 20(9) |
| Pages | 748-54 |
| Published | 2006-9-1 |
| DOI | 10.1002/ptr.1947 |
| PMID | 16807878 |
| MeSH | Animals Cell Line, Tumor Ephedra sinica* Gene Expression / drug effects Melanins / biosynthesis* Melanocytes / drug effects* Mice Microphthalmia-Associated Transcription Factor / metabolism Monophenol Monooxygenase / metabolism Oligonucleotide Array Sequence Analysis Plant Extracts / pharmacology* Reverse Transcriptase Polymerase Chain Reaction |
| IF | 4.087 |
| Times Cited | 11 |
| WOS Category | PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL |
| Resource | |
| Human and Animal Cells | |