論文 - 詳細
| RRC ID | 42688 |
|---|---|
| 著者 | Hansman GS, Oka T, Katayama K, Takeda N. |
| タイトル | Enhancement of sapovirus recombinant capsid protein expression in insect cells. |
| ジャーナル | FEBS Lett |
| Abstract |
Human sapovirus (SaV) is uncultivable, but expression of the recombinant capsid protein (rVP1) in insect cells results in the formation of virus-like particles (VLPs) that are morphologically similar to the native viruses. However, the SaV rVP1 expression levels are considerably low. We have found that inclusions of short foreign nucleotide sequences inserted directly upstream from the predicted rVP1 AUG start codon lead to increased yield of VLPs. This method allowed us to express a SaV rVP1, which could not have been expressed to measurable or practical levels otherwise. |
| 巻・号 | 580(17) |
| ページ | 4047-50 |
| 公開日 | 2006-7-24 |
| DOI | 10.1016/j.febslet.2006.06.040 |
| PII | S0014-5793(06)00747-2 |
| PMID | 16814288 |
| MeSH | Animals Capsid / ultrastructure Capsid Proteins / biosynthesis* Capsid Proteins / genetics Cell Line Codon, Initiator Gene Expression Humans Infant, Newborn Male Mutagenesis, Insertional Recombinant Proteins / biosynthesis* Recombinant Proteins / genetics Sapovirus* / genetics Sapovirus* / ultrastructure Spodoptera / cytology |
| IF | 3.057 |
| 引用数 | 7 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Sf9 |