論文 - 詳細
| RRC ID | 42708 |
|---|---|
| 著者 | Yasuda S, Itagaki S, Hirano T, Iseki K. |
| タイトル | Effects of sex hormones on regulation of ABCG2 expression in the placental cell line BeWo. |
| ジャーナル | J Pharm Pharm Sci |
| Abstract |
PURPOSE:The aim of this study was to elucidate the effects of sex hormones that are secreted during gestation from the placenta on ABCG2 mRNA and protein expression levels by using the placental cell line BeWo. METHODS:We investigated the effects of estrogens (estrone, 17-beta-estradiol and estriol) on the expression level of ABCG2 mRNA by RT-PCR. The expression level of ABCG2 protein was analyzed by Western blot analysis. We also investigated the localization of ABCG2 in BeWo cells by Western blot analysis of the plasma membrane fraction and by immunohistochemistry. RESULTS:It was found that all estrogens induce the expression of ABCG2 mRNA in a concentration-dependent manner. Furthermore, Western blot analysis showed that 17-beta-estradiol induces the expression of ABCG2 protein. Western blot analysis of the plasma membrane fraction and immunohistochemistry showed that ABCG2 localized on only the apical side of BeWo cells and that 17-beta-estradiol had no effect on the localization of ABCG2. In addition, progesterone suppressed the induction of ABCG2 expression by 17-beta-estradiol at 1-10 microM. CONCLUSION:The expression of ABCG2 in the placenta is regulated by estrogen and progesterone during gestation. |
| 巻・号 | 9(1) |
| ページ | 133-9 |
| 公開日 | 2006-1-1 |
| PMID | 16849015 |
| MeSH | ATP Binding Cassette Transporter, Subfamily G, Member 2 ATP-Binding Cassette Transporters / genetics ATP-Binding Cassette Transporters / metabolism* Cell Line Cell Line, Tumor Estrogens / pharmacology Gene Expression / drug effects* Gene Expression / physiology Gonadal Steroid Hormones / pharmacology* Humans Neoplasm Proteins / genetics Neoplasm Proteins / metabolism* Placenta / pathology* |
| IF | 1.667 |
| 引用数 | 20 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | BeWo(RCB1644) |