Reference - Detail
| RRC ID | 42716 |
|---|---|
| Author | Lee KH, Choi EM. |
| Title | Rubus coreanus Miq. extract promotes osteoblast differentiation and inhibits bone-resorbing mediators in MC3T3-E1 cells. |
| Journal | Am J Chin Med |
| Abstract |
To prevent bone loss that occurs with increasing age, certain nutritional and pharmacological factors are needed. In the present study, the ethanol extract from the fruit of Rubus coreanus Miq. (RCE) was investigated for its effect on the function of osteoblastic MC3T3-E1 cells. RCE (10approximately50 microg/ml) caused a significant elevation in cell viability, alkaline phosphatase (ALP) activity, collagen content, and osteocalcin secretion in the cells. The effect of RCE (50 microg/ml) in increasing cell viability, ALP activity, and collagen content was prevented by the presence of 10(-6) M cycloheximide and 10(-6) M tamoxifen, suggesting that RCE's effect results from a newly synthesized protein component and might be partly involved in estrogen action. We then examined the effect of RCE on the H(2)O(2)-induced apoptosis and production of local factors in osteoblasts. Treatment with RCE (10approximately50 microg/ml) decreased the 0.2 mM H(2)O(2)-induced apoptosis and production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6 and nitric oxide (NO) in osteoblasts. Our data indicate that the enhancement of osteoblast function by Rubus coreanus Miq. may result in the prevention of osteoporosis and inflammatory bone diseases. |
| Volume | 34(4) |
| Pages | 643-54 |
| Published | 2006-1-1 |
| DOI | 10.1142/S0192415X0600417X |
| PII | S0192415X0600417X |
| PMID | 16883635 |
| MeSH | 3T3 Cells Alkaline Phosphatase / metabolism Animals Apoptosis / drug effects Cell Differentiation / drug effects* Cell Survival / drug effects Collagen / biosynthesis Cycloheximide / pharmacology Cytokines / biosynthesis Dose-Response Relationship, Drug Fruit / chemistry Hydrogen Peroxide / toxicity Mice Nitric Oxide / biosynthesis Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Osteocalcin / metabolism Plant Extracts / pharmacology* Rosaceae / chemistry* Tamoxifen / pharmacology |
| IF | 3.682 |
| Times Cited | 19 |
| WOS Category | INTEGRATIVE & COMPLEMENTARY MEDICINE |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |