RRC ID 42728
Author Nakatani S, Mano H, Ryanghyok IM, Shimizu J, Wada M.
Title Excess magnesium inhibits excess calcium-induced matrix-mineralization and production of matrix gla protein (MGP) by ATDC5 cells.
Journal Biochem Biophys Res Commun
Abstract We found that excessive extracellular Ca2+ and/or Mg2+ affected the process of matrix mineralization and glycosaminoglycan (GAG) production by cells of the prechondrogenic cell line, ATDC5. Excess Ca2+ induced both matrix mineralization and GAG production in the cells. On the other hand, excess Mg2+ reduced this Ca2+-mediated rise in both mineralization and GAG production in them. Next we measured the mRNA levels of cartilage-associated genes such as calcium-sensing receptor (CaSR), matrix gla protein (MGP), bone gla protein (BGP), and Runt-related transcription factor 2 (Runx2) in ATDC5 cells. Excess Ca2+ increased the MGP, BGP, and CaSR mRNA levels, and excess Mg2+ reduced the Ca2+-induced increase in the MGP mRNA level in the cells. The changes in the MGP mRNA level paralleled those in the MGP protein level. These data show that Ca2+ and Mg2+ regulated the matrix mineralization positively and negatively, respectively, in ATDC5 cells and suggest that excess Mg2+ might inhibit the excess Ca2+-promoted mineralization mediated by MGP induction in chondrocytes.
Volume 348(3)
Pages 1157-62
Published 2006-9-29
DOI 10.1016/j.bbrc.2006.07.180
PII S0006-291X(06)01753-0
PMID 16904632
MeSH Animals Calcium / antagonists & inhibitors Calcium / physiology* Calcium-Binding Proteins / antagonists & inhibitors* Calcium-Binding Proteins / biosynthesis* Cell Line, Tumor Extracellular Matrix / metabolism* Extracellular Matrix Proteins / antagonists & inhibitors* Extracellular Matrix Proteins / biosynthesis* Glycosaminoglycans / biosynthesis Humans Magnesium / metabolism* Magnesium / physiology Mice
IF 2.985
Times Cited 26
Human and Animal Cells ATDC5(RCB0565) 293T(RCB2202)