論文 - 詳細
| RRC ID | 42731 |
|---|---|
| 著者 | Lee KH, Choi EM. |
| タイトル | Stimulatory effects of extract prepared from the bark of Cinnamomum cassia blume on the function of osteoblastic MC3T3-E1 cells. |
| ジャーナル | Phytother Res |
| Abstract |
The ethanol extract from the bark of Cinnamomum cassia Blume (CCE) was tested for estrogenic activity. CCE (4-60 microg/mL) significantly induced the growth of MCF-7 cells, an ER-positive human breast cancer cell line, over that of untreated control cells (p < 0.05). In the ER competitive binding assay, CCE showed higher affinity with ERbeta compared with ERalpha. To investigate the bioactivities of CCE, which act on bone metabolism, the effects of CCE on the function of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts were studied. CCE (4-60 microg/mL) dose-dependently increased the survival of MC3T3-E1 cells. In addition, CCE (10 and 50 microg/mL) increased alkaline phosphatase (ALP) activity, collagen synthesis and osteocalcin secretion in MC3T3-E1 cells. Treatment with CCE (10 and 50 microg/mL) prevented apoptosis induced by TNF-alpha (10(-10) m) in osteoblastic cells. In the presence of TNF-alpha, culture with CCE (10-100 microg/mL) for 48 h inhibited the production of IL-6 and nitric oxide in osteoblastic MC3T3-E1 cells. These results suggest that Cinnamomum cassia has a direct stimulatory effect on bone formation in vitro and may contribute to the prevention of osteoporosis and inflammatory bone diseases. |
| 巻・号 | 20(11) |
| ページ | 952-60 |
| 公開日 | 2006-11-1 |
| DOI | 10.1002/ptr.1984 |
| PMID | 16906639 |
| MeSH | Alkaline Phosphatase / drug effects Animals Apoptosis / drug effects Cell Line, Tumor Cinnamomum aromaticum / chemistry* Collagen / analysis Estrogens / pharmacology* Humans Inhibitory Concentration 50 Interleukin-6 / analysis Mice Nitric Oxide / analysis Osteoblasts / drug effects* Osteocalcin / analysis Plant Bark / chemistry Plant Extracts / pharmacology* Survival Analysis Tumor Necrosis Factor-alpha / pharmacology |
| IF | 4.087 |
| 引用数 | 16 |
| WOS 分野 | PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |