| RRC ID |
42743
|
| Author |
Matsubara T, Myoui A, Ikeda F, Hata K, Yoshikawa H, Nishimura R, Yoneda T.
|
| Title |
Critical role of cortactin in actin ring formation and osteoclastic bone resorption.
|
| Journal |
J Bone Miner Metab
|
| Abstract |
Tyrosine kinase c-Src plays an essential role in ruffled border formation and bone resorption in osteoclasts; however, it is unclear how c-Src controls ruffled border formation during bone resorption. To address this question, we investigated the role of cortactin, a c-Src substrate, in osteoclasts. We found that cortactin showed colocalization with c-Src and actin rings in osteoclasts. Overexpression of cortactin stimulated actin ring formation in RAW 264.7 cells. In contrast, overexpression of Csk inhibited tyrosine phosphorylation of cortactin and binding of cortactin to c-Src. More importantly, overexertion of a mutant cortactin strongly suppressed actin ring formation and bone resorbing activity in osteoclasts. Collectively, our data indicate that cortactin controls osteoclastic bone resorption by regulating actin organization.
|
| Volume |
24(5)
|
| Pages |
368-72
|
| Published |
2006-1-1
|
| DOI |
10.1007/s00774-006-0701-4
|
| PMID |
16937268
|
| MeSH |
Actins / metabolism*
Animals
Bone Resorption / metabolism*
Cell Line
Cortactin / physiology*
Humans
Mice
Mice, Inbred C57BL
Monocytes / physiology
Osteoclasts / physiology*
Proto-Oncogene Proteins pp60(c-src) / physiology
|
| IF |
2.297
|
| Times Cited |
18
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
ENDOCRINOLOGY & METABOLISM
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
