RRC ID |
42747
|
Author |
Hirasawa N, Torigoe M, Kano K, Ohuchi K.
|
Title |
Involvement of Sp1 in lipopolysaccharide-induced expression of HDC mRNA in RAW 264 cells.
|
Journal |
Biochem Biophys Res Commun
|
Abstract |
The involvement of Sp1 in the lipopolysaccharide (LPS)-induced transcription of HDC mRNA in the mouse macrophage-like cell line RAW 264 was analyzed. LPS increased the levels of HDC mRNA 4 h after the stimulation in a concentration-dependent manner. Mithramycin A, an inhibitor of the binding of the Sp family to the GC box, reduced the LPS-induced increase in the levels of HDC mRNA at 4 h and HDC protein at 8 h in a concentration-dependent manner. By conducting electrophoretic mobility shift assays, we found that one of the transcription factors binding to the DNA probe containing the GC box sequence of the mouse HDC gene promoter region was Sp1, and that levels of Sp1-DNA probe complexes were increased by stimulation with LPS although the protein levels of Sp1 were not changed. These results suggested that Sp1 is one of the transcription factors that regulate the LPS-induced expression of HDC in RAW 264 cells.
|
Volume |
349(2)
|
Pages |
833-7
|
Published |
2006-10-20
|
DOI |
10.1016/j.bbrc.2006.08.104
|
PII |
S0006-291X(06)01930-9
|
PMID |
16949047
|
MeSH |
Animals
Cell Line
Cell Nucleus / metabolism
Dose-Response Relationship, Drug
Histidine Decarboxylase / chemistry*
Histidine Decarboxylase / metabolism
Lipopolysaccharides / metabolism*
Lipopolysaccharides / pharmacology
Macrophages / metabolism
Mast Cells / metabolism
Mice
Plicamycin / analogs & derivatives
Plicamycin / pharmacology
Promoter Regions, Genetic
RNA, Messenger / metabolism
Sp1 Transcription Factor / chemistry
Sp1 Transcription Factor / physiology*
Time Factors
|
IF |
2.985
|
Times Cited |
12
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Human and Animal Cells |
RAW 264(RCB0535) |