| RRC ID |
42749
|
| Author |
Okano T, Nakagawa T, Kita T, Endo T, Ito J.
|
| Title |
Cell-gene delivery of brain-derived neurotrophic factor to the mouse inner ear.
|
| Journal |
Mol Ther
|
| Abstract |
Sensorineural hearing loss is a common disability, but treatment options are currently limited to cochlear implants and hearing aids. Studies are therefore being conducted to provide alternative means of biological therapy, including gene therapy. Safe and effective methods of gene delivery to the cochlea need to be developed to facilitate the clinical application of these therapeutic treatments for hearing loss. In this study, we examined the potential of cell-gene therapy with nonviral vectors for delivery of therapeutic molecules into the cochlea. NIH3T3 cells were transfected with the brain-derived neurotrophic factor (Bdnf) gene using lipofection and then transplanted into the mouse inner ear. Immunohistochemistry and Western blotting demonstrated the survival of grafted cells in the cochlea for up to 4 weeks after transplantation. No significant hearing loss was induced by the transplantation procedure. A Bdnf-specific enzyme-linked immunosorbent assay revealed a significant increase in Bdnf production in the inner ear following transplantation of engineered cells. These findings indicate that cell-gene delivery with nonviral vectors may be applicable for the local, sustained delivery of therapeutic molecules into the cochlea.
|
| Volume |
14(6)
|
| Pages |
866-71
|
| Published |
2006-12-1
|
| DOI |
10.1016/j.ymthe.2006.06.012
|
| PII |
S1525-0016(06)00264-4
|
| PMID |
16956795
|
| MeSH |
Animals
Auditory Threshold
Blotting, Western
Brain-Derived Neurotrophic Factor / genetics*
Brain-Derived Neurotrophic Factor / metabolism
Cell Transplantation / methods*
Cochlea / cytology
Cochlea / metabolism
Ear, Inner / cytology
Ear, Inner / metabolism*
Ear, Inner / surgery
Graft Survival / genetics
Graft Survival / physiology
Immunohistochemistry
Male
Mice
Mice, Inbred C57BL
NIH 3T3 Cells
RNA, Messenger / genetics
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transfection / methods
|
| IF |
8.986
|
| Times Cited |
28
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| Resource |
| Human and Animal Cells |
NIH3T3-3(RCB0150) |
