RRC ID 42750
著者 Uemori A, Maeda M, Yasutake K, Ohgushi A, Kagoshima K, Mizuki E, Ohba M.
タイトル Ubiquity of parasporin-1 producers in Bacillus thuringiensis natural populations of Japan.
ジャーナル Naturwissenschaften
Abstract Parasporin, a Bacillus thuringiensis parasporal protein, is unique in having a strong cytocidal activity preferential for human cancer cells. In this study, we characterized parasporin activities associated with three novel geographical isolates of B. thuringiensis. Parasporal inclusion proteins of the three isolates were highly toxic to human uterus cervix cancer cells (HeLa), but not to non-cancer uterine smooth muscle cells (UtSMC). Inclusions of the isolates lacked insect toxicity and hemolytic activity against sheep erythrocytes. Ouchterlony immunodiffusion tests revealed that the proteins of the three isolates are immunologically closely related to parasporin-1 (Cry31A), but dissimilar to the three other existing parasporin groups. Our results provide evidence that the parasporin-1-producing organism is a common member in B. thuringiensis populations occurring in natural environments of Japan.
巻・号 94(1)
ページ 34-8
公開日 2007-1-1
DOI 10.1007/s00114-006-0153-7
PMID 16957922
MeSH Agglutination Tests Antineoplastic Agents / therapeutic use Antineoplastic Agents / toxicity Bacillus thuringiensis / physiology* Bacillus thuringiensis / ultrastructure Bacterial Proteins / biosynthesis Bacterial Proteins / therapeutic use Bacterial Proteins / toxicity Endotoxins / biosynthesis* Endotoxins / therapeutic use Endotoxins / toxicity HeLa Cells / drug effects Humans Japan Neoplasms / drug therapy
IF 2.09
引用数 16
WOS 分野 MICROBIOLOGY
リソース情報
ヒト・動物細胞