RRC ID 42758
Author Yoshida T, Asanuma M, Grossmann L, Fuse M, Shibata T, Yonekawa T, Tanaka T, Ueno K, Yasuda T, Saito Y, Tatsuno I.
Title Geranylgeranyl-pyrophosphate (GGPP) synthase is down-regulated during differentiation of osteoblastic cell line MC3T3-E1.
Journal FEBS Lett
Abstract Isoprenylation of geranylgeranyl-pyrophosphate (GGPP) is critical for activation of small GTPases. We examined the roles of GGPP synthase (GGPPS) during the differentiation induced by the cell-to-cell contact in osteoblastic cell line MC3T3-E1 cells. We found that (1) both mRNA and protein expression of GGPPS was reduced with decrement of its activity during the differentiation, (2) GGOH, which is converted to GGPP in the cells, inhibited differentiation. These results suggest that the decrement of GGPP is critical for the cell-to-cell contact-induced differentiation, in which the down-regulation of GGPPS might be involved.
Volume 580(22)
Pages 5203-7
Published 2006-10-2
DOI 10.1016/j.febslet.2006.08.060
PII S0014-5793(06)01033-7
PMID 16978613
MeSH Animals Cell Communication / physiology* Cell Differentiation / physiology* Cell Line Down-Regulation / physiology* Enzyme Activation / physiology Farnesyltranstransferase / biosynthesis* Farnesyltranstransferase / genetics Gene Expression Regulation, Enzymologic / physiology* Mice Monomeric GTP-Binding Proteins / genetics Monomeric GTP-Binding Proteins / metabolism Multienzyme Complexes / biosynthesis* Multienzyme Complexes / genetics Osteoblasts / cytology Osteoblasts / enzymology* RNA, Messenger / biosynthesis RNA, Messenger / genetics
IF 3.057
Times Cited 17
Human and Animal Cells MC3T3-E1(RCB1126)