Reference - Detail
|Author||Yoshida T, Asanuma M, Grossmann L, Fuse M, Shibata T, Yonekawa T, Tanaka T, Ueno K, Yasuda T, Saito Y, Tatsuno I.|
|Title||Geranylgeranyl-pyrophosphate (GGPP) synthase is down-regulated during differentiation of osteoblastic cell line MC3T3-E1.|
Isoprenylation of geranylgeranyl-pyrophosphate (GGPP) is critical for activation of small GTPases. We examined the roles of GGPP synthase (GGPPS) during the differentiation induced by the cell-to-cell contact in osteoblastic cell line MC3T3-E1 cells. We found that (1) both mRNA and protein expression of GGPPS was reduced with decrement of its activity during the differentiation, (2) GGOH, which is converted to GGPP in the cells, inhibited differentiation. These results suggest that the decrement of GGPP is critical for the cell-to-cell contact-induced differentiation, in which the down-regulation of GGPPS might be involved.
|MeSH||Animals Cell Communication / physiology* Cell Differentiation / physiology* Cell Line Down-Regulation / physiology* Enzyme Activation / physiology Farnesyltranstransferase / biosynthesis* Farnesyltranstransferase / genetics Gene Expression Regulation, Enzymologic / physiology* Mice Monomeric GTP-Binding Proteins / genetics Monomeric GTP-Binding Proteins / metabolism Multienzyme Complexes / biosynthesis* Multienzyme Complexes / genetics Osteoblasts / cytology Osteoblasts / enzymology* RNA, Messenger / biosynthesis RNA, Messenger / genetics|
|WOS Category||BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|
|Human and Animal Cells|