| RRC ID |
42791
|
| Author |
Ninomiya M, Abe A, Katsumi A, Xu J, Ito M, Arai F, Suda T, Ito M, Kiyoi H, Kinoshita T, Naoe T.
|
| Title |
Homing, proliferation and survival sites of human leukemia cells in vivo in immunodeficient mice.
|
| Journal |
Leukemia
|
| Abstract |
The cellular components of the hematopoietic stem cell niche have been gradually identified. However, the niche for malignant hematopoiesis remains to be elucidated. Here, using human leukemia cells, which could be transplanted to immunodeficient mice, we studied the in vivo homing, proliferation and survival sites by immunohistopathology, compared with the corresponding sites for cord blood CD34(+) (CBCD34(+)) cells. The human leukemia cells initially localized on the surface of osteoblasts in the epiphysial region, and expanded to the inner vascular and diaphysial regions within 4 weeks. The percentage of CD34(+) leukemia cells in the bone marrow was transiently increased up to 50%. In vivo 5-bromo-2'-deoxyuridine labeling revealed that the epiphysis was the most active site for leukemia cell proliferation. CBCD34(+) cells showed the similar pattern of homing and proliferation to leukemia cells. After high-dose administration of cytosine-1-beta-D-arabinofuranoside, residual leukemia cells were localized in the perivascular endothelium as well as in contact with the trabecular endosteum. These findings suggest that xenotransplantation into immunodeficient mice provides a useful model to study the leukemia niche.
|
| Volume |
21(1)
|
| Pages |
136-42
|
| Published |
2007-1-1
|
| DOI |
10.1038/sj.leu.2404432
|
| PII |
2404432
|
| PMID |
17039228
|
| MeSH |
Animals
Antigens, CD34
Arabinonucleosides / pharmacology
Bromodeoxyuridine
Cell Movement
Cell Proliferation
Cell Survival
Cord Blood Stem Cell Transplantation
Hematopoietic Stem Cells / pathology
Humans
Leukemia / pathology*
Leukocyte Common Antigens
Mice
Mice, Inbred NOD
Mice, SCID
Neoplasm Transplantation
Tumor Cells, Cultured
|
| IF |
8.665
|
| Times Cited |
69
|
|
WOS Category
|
ONCOLOGY
HEMATOLOGY
|
| Resource |
| Human and Animal Cells |
KG-1(RCB1166) |
