RRC ID 42802
Author Chan CB, Tang WK, Cheng CH, Fong WP.
Title Cloning of the black seabream (Acanthopagrus schlegeli) antiquitin gene and functional characterization of its promoter region.
Journal Mol Cell Biochem
Abstract Antiquitin (ALDH7) is a member of the aldehyde dehydrogenase superfamily. In plants, ALDH7 is inducible upon dehydration and is thus believed to possess an osmoregulatory role. On the other hand, however, its exact physiological function in animals remains elusive. We herein report the isolation of the black seabream (Acanthopagrus schlegeli) antiquitin gene (sbALDH7) and the functional characterization of its promoter region. The 1.6 kb 5'-flanking region of sbALDH7 exhibits an intense promoter activity (30-170 fold of the basal) in five mammalian and fish cell lines of different origins. Progressive 5'-deletion analysis suggests that the core promoter is located within the region -297/+41 whereas a cis-acting repressor of basal transcription is present in the region -878/-297. In silico analysis of this sbALDH7 promoter region does not reveal any osmotic response element. Instead, it contains potential binding sites for cell cycle related cis-elements such as CCAAT displacement protein and cell cycle-dependent element/cell cycle genes homology region.
Volume 297(1-2)
Pages 151-60
Published 2007-3-1
DOI 10.1007/s11010-006-9340-2
PMID 17075685
MeSH 5' Flanking Region / genetics Animals Base Sequence Binding Sites / genetics CHO Cells Cloning, Molecular Cricetinae Cricetulus Exons / genetics Fish Proteins / genetics* Humans Molecular Sequence Data Promoter Regions, Genetic / genetics* Sea Bream / genetics* Sequence Analysis, DNA Sequence Deletion Transcription Initiation Site
IF 2.795
Times Cited 2
Human and Animal Cells